ArticleMolecular & general genetics : MGG1993
Expression of the Saccharomyces cerevisiae RAD50 gene during meiosis: steady-state transcript levels rise and fall while steady-state protein levels remain constant.
Article in Molecular & general genetics : MGG, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- IpO: plasmids and methods for simplified, PCR-based DNA transplant in yeast.Yeast (Chichester, England) · 2014Article
- Regulation of Saccharomyces cerevisiae DNA polymerase eta transcript and protein.Radiation and environmental biophysics · 2008Article
- An mre11 mutant of Coprinus cinereus has defects in meiotic chromosome pairing, condensation and synapsis.Genetics · 2000Article
- The rad9 gene of Coprinus cinereus encodes a proline-rich protein required for meiotic chromosome condensation and synapsis.Genetics · 1996Article
- Promoter elements of the PHR1 gene of Saccharomyces cerevisiae and their roles in the response to DNA damage.Nucleic acids research · 1995Article
- Mutations in XRS2 and RAD50 delay but do not prevent mating-type switching in Saccharomyces cerevisiae.Molecular and cellular biology · 1994Article
- Control of meiotic gene expression in Saccharomyces cerevisiae.Microbiological reviews · 1994Review
- RAD50 protein of S.cerevisiae exhibits ATP-dependent DNA binding.Nucleic acids research · 1993Article
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2 authors at 1 institution in 1 country.
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Abstract
In Saccharomyces cerevisiae, the RAD50 gene is required for repair of X-ray and MMS-induced DNA damage during vegetative growth, and for synaptonemal complex formation and genetic recombination during meiosis. We show below that the RAD50 gene encodes major and minor transcripts of 4.2 and 4.6 kb in length which differ primarily at their 5' ends. Steady-state levels of both RAD50 transcripts increase coordinately during meiosis, reaching maximal levels midway through meiotic prophase, about 3 or 4 h after transfer of cells to sporulation medium. The 5' ends of the major RAD50 transcript in both meiotic and vegetative cells map to the same cluster of sites approximately 20 bp upstream of the amino-terminal ATG of the RAD50 coding sequence. We conclude that the increased RAD50 transcript level observed during meiosis does not reflect utilization of a new promoter. In contrast, steady-state levels of Rad50 protein do not increase during meiosis. Thus, changes in RAD50 transcript levels are not necessarily accompanied by commensurate changes in Rad50 protein levels. Possible explanations are considered.
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