ArticleThe Journal of cell biology1993
Expression of chicken vinculin complements the adhesion-defective phenotype of a mutant mouse F9 embryonal carcinoma cell.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- A Novel Method in Identifying Pyroptosis and Apoptosis Based on the Double Resonator Piezoelectric Cytometry Technology.Biosensors · 2023Article
- Vinculin and talin: focus on the myocardium.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2009Review
- alpha-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells.The Journal of cell biology · 1998Article
- Attachment kinetics, proliferation rates and vinculin assembly of bovine osteoblasts cultured on different pre-coated artificial substrates.Journal of materials science. Materials in medicine · 1998Article
- Characterization of cell-matrix adhesion requirements for the formation of fascin microspikes.Molecular biology of the cell · 1997Article
- Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding.The Journal of cell biology · 1996Article
- Talin and vinculin play distinct roles in filopodial motility in the neuronal growth cone.The Journal of cell biology · 1996Article
- Hisactophilin-mediated binding of actin to lipid lamellae: a neutron reflectivity study of protein membrane coupling.Biophysical journal · 1996Article
- Tumor autocrine motility factor responses are mediated through cell contact and focal adhesion rearrangement in the absence of new tyrosine phosphorylation in metastatic cells.The American journal of pathology · 1996Article
- Vinculin, talin and focal adhesions.Journal of muscle research and cell motility · 1996Review
- Detachment of cultured cells from the substratum induced by the neutrophil-derived oxidant NH2Cl: synergistic role of phosphotyrosine and intracellular Ca2+ concentration.The Journal of cell biology · 1995Article
- Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Interaction of the 47-kDa talin fragment and the 32-kDa vinculin fragment with acidic phospholipids: a computer analysis.Biophysical journal · 1995Article
- The essential yeast Tcp1 protein affects actin and microtubules.Molecular biology of the cell · 1994Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A mutant cell line, derived from the mouse embryonal carcinoma cell line F9, is defective in cell-cell adhesion (compaction) and in cell-substrate adhesion. We have previously shown that neither uvomorulin (E-cadherin) nor integrins are responsible for the mutant phenotype (Calogero, A., M. Samuels, T. Darland, S. A. Edwards, R. Kemler, and E. D. Adamson. 1991. Dev. Biol. 146:499-508). Several cytoskeleton proteins were assayed and only vinculin was found to be absent in mutant (5.51) cells. A chicken vinculin expression vector was transfected into the 5.51 cells together with a neomycin-resistance vector. Clones that were adherent to the substrate were selected in medium containing G418. Two clones, 5.51Vin3 and Vin4, were analyzed by Nomarski differential interference contrast and laser confocal microscopy as well as by biochemical and molecular biological techniques. Both clones adhered well to substrates and both exhibited F-actin stress fibers with vinculin localized at stress fiber tips in focal contacts. This was in marked contrast to 5.51 parental cells, which had no stress fibers and no vinculin. The mutant and complemented F9 cell lines will be useful models for examining the complex interactions between cytoskeletal and cell adhesion proteins.
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