ArticleThe Histochemical journal1993
Immunolocalization of manganese superoxide dismutase in normal and transgenic mice expressing the human enzyme.
Article in The Histochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Physiological and pathophysiological role of reactive oxygen species and reactive nitrogen species in the kidney.Clinical and experimental pharmacology & physiology · 2018Review
- Oxidant Mechanisms in Renal Injury and Disease.Antioxidants & redox signaling · 2016Review
- Paradoxical Roles of Antioxidant Enzymes: Basic Mechanisms and Health Implications.Physiological reviews · 2016Review
- Oxidative stress in hypertension: role of the kidney.Antioxidants & redox signaling · 2014Review
- Protective effect of probucol on liver injury induced by carbon tetrachloride in rats.Hepatology international · 2011Article
- Chemistry and antihypertensive effects of tempol and other nitroxides.Pharmacological reviews · 2008Review
- Hippocampal long-term potentiation, memory, and longevity in mice that overexpress mitochondrial superoxide dismutase.Neurobiology of learning and memory · 2007Article
- Manganese superoxide dismutase protects against 6-hydroxydopamine injury in mouse brains.The Journal of biological chemistry · 2005Article
- Glucose transporters in diabetic nephropathy.Pediatric nephrology (Berlin, Germany) · 2005Review
- Glucose transporter-1-deficient mice exhibit impaired development and deformities that are similar to diabetic embryopathy.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The nature of antioxidant defense mechanisms: a lesson from transgenic studies.Environmental health perspectives · 1998Review
- Immunodetection of manganese superoxide dismutase in cultured human retroocular fibroblasts using sera directed against the thyrotropin receptor.Journal of endocrinological investigation · 1998Article
- Antioxidant enzymes in the human iris: an immunogold study.The British journal of ophthalmology · 1997Article
- Immunohistochemical localization of antioxidant enzymes during hamster kidney development.The Histochemical journal · 1995Article
- Immunolocalization of antioxidant enzymes in adult hamster kidney.The Histochemical journal · 1994Article
- Immunogold analysis of antioxidant enzymes in human renal cell carcinoma.Virchows Archiv : an international journal of pathology · 1994Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
The localization of manganese superoxide dismutase (MnSOD) was determined using immunohistochemistry of various tissues of normal and transgenic mice which express the human enzyme, with emphasis on studies of mouse kidney and lung. Mouse kidney and lung were studied using both frozen section analysis and paraffin sections following fixation in a variety of fixatives. Formalin fixation resulted in a loss of antigenicity, while fixation in zinc formalin or B5 fixative gave results similar to those from frozen sections. Immunoperoxidase studies using antibodies to MnSOD showed greater staining in transgenic kidney or lung than in identical tissues in normal mice when appropriate fixation was used. In contrast, equal immunostaining was obtained in kidney or lung from normal and transgenic mice when antibodies to catalase or copper zinc superoxide dismutase were utilized. Immunogold ultrastructural analysis of MnSOD localization for lung and kidney was also performed. As compared to normal mice, transgenic mice exhibited greater staining of the mitochondria of kidney interstitial fibroblasts and glomerular, endothelial, and smooth muscle cells. In the lungs of transgenic animals, all cells showed increased staining; smooth muscle cells demonstrated the most marked increase in immunolabelling. The results indicate that these transgenic mice overexpress MnSOD in their mitochondria, and that this occurs selectively in at least some mesenchymal tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.