ArticleThe EMBO journal1993
Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 142 citations in OpenAlex.
- α-glucan remodeling by GH13-domain enzymes shapes fungal cell wall architecture.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- The Fission Yeast Cell Integrity Pathway: A Functional Hub for Cell Survival upon Stress and Beyond.Journal of fungi (Basel, Switzerland) · 2021Review
- Review
- Fission yeast cell wall biosynthesis and cell integrity signalling.Cell surface (Amsterdam, Netherlands) · 2018Review
- Protein kinase C is essential for viability of the rice blast fungus Magnaporthe oryzae.Molecular microbiology · 2015Article
- Cell migration and division in amoeboid-like fission yeast.Biology open · 2014Article
- The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway.MicrobiologyOpen · 2013Article
- Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring.The Journal of cell biology · 2011Article
- The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast.Molecular biology of the cell · 2009Article
- Atf1 is a target of the mitogen-activated protein kinase Pmk1 and regulates cell integrity in fission yeast.Molecular biology of the cell · 2007Article
- Rho2 is a target of the farnesyltransferase Cpp1 and acts upstream of Pmk1 mitogen-activated protein kinase signaling in fission yeast.Molecular biology of the cell · 2006Article
- Real-time monitoring of calcineurin activity in living cells: evidence for two distinct Ca2+-dependent pathways in fission yeast.Molecular biology of the cell · 2006Article
- Protein kinase C (PkcA) of Aspergillus nidulans is involved in penicillin production.Applied and environmental microbiology · 2006Article
- Rgf1p is a specific Rho1-GEF that coordinates cell polarization with cell wall biogenesis in fission yeast.Molecular biology of the cell · 2006Article
- Article
- Evolution, biochemistry and genetics of protein kinase C in fungi.Current genetics · 2003Review
- Fission yeast Mor2/Cps12, a protein similar to Drosophila Furry, is essential for cell morphogenesis and its mutation induces Wee1-dependent G(2) delay.The EMBO journal · 2002Article
- Schizosaccharomyces pombe rho2p GTPase regulates cell wall alpha-glucan biosynthesis through the protein kinase pck2p.Molecular biology of the cell · 2000Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two novel protein kinase C (PKC)-like genes, pck1+ and pck2+ were isolated from fission yeast by PCR. Both contain common domains of PKC-related molecules, but lack a putative Ca(2+)-binding domain so that they may belong to the nPKC group. Gene disruption of pck1+ and pck2+ establishes that they share an overlapping essential function for cell viability. Cells of a single pck2 deletion display severe defects in cell shape; they are irregular and sometimes pear-like instead of cylindrical. In contrast, the induced overexpression of pck2+ is lethal, producing multiseptated and branched cells. These results suggest that fission yeast PKC-like genes are involved in the polarity of cell growth control. We show that pck2 is allelic to sts6, a locus we have previously identified by its supersensitivity to staurosporine, a potent protein kinase inhibitor [Toda et al. (1991) Genes Dev., 5, 60-73]. In addition, the lethal overexpression of pck2+ can be suppressed by staurosporine, indicating that fission yeast pck1 and pck2 are molecular targets of this inhibitor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.