ArticleThe Biochemical journal1993
Identification, purification and characterization of a membrane-associated N-myristoyltransferase inhibitor protein from bovine brain.
Article in The Biochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 45 citations in OpenAlex.
- The Cellular Senescence-Inhibited Gene Is Essential for PPM1A Myristoylation To Modulate Transforming Growth Factor β Signaling.Molecular and cellular biology · 2018Article
- Protein myristoylation in health and disease.Journal of chemical biology · 2010Article
- Tris (dibenzylideneacetone) dipalladium, a N-myristoyltransferase-1 inhibitor, is effective against melanoma growth in vitro and in vivo.Clinical cancer research : an official journal of the American Association for Cancer Research · 2008Article
- Copper(II) and manganese(III) complexes of N'-[(2-hydroxy phenyl) carbonothioyl] pyridine-2-carbohydrazide: novel therapeutic agents for cancer.Biochimie · 2006Article
- Biological significance of phosphorylation and myristoylation in the regulation of cardiac muscle proteins.Molecular and cellular biochemistry · 1997Review
- Coenzyme A dependent myristoylation and demyristoylation in the regulation of bovine spleen N-myristoyltransferase.Molecular and cellular biochemistry · 1996Article
- Expression of human N-myristoyltransferase in Escherichia coli. Comparison with N-myristoyltransferases expressed in different tissues.Molecular and cellular biochemistry · 1996Article
- Mechanisms of action of NIP71 on N-myristoyltransferase activity.Molecular and cellular biochemistry · 1994Article
- In vivo modulation of N-myristoyltransferase activity by orthovanadate.Molecular and cellular biochemistryArticle
- Expression and activity of N-myristoyltransferase in lung inflammation of cattle and its role in neutrophil apoptosis.Veterinary researchArticle
- Mammalian myristoyl CoA: protein N-myristoyltransferase.Molecular and cellular biochemistryArticle
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
N-Myristoyl-CoA: protein N-myristoyltransferase (NMT) is the enzyme that catalyses the covalent transfer of myristic acid from myristoyl-CoA to the N-terminal glycine residue of a protein substrate. Subcellular fractionation of bovine brain indicates that NMT activity was located in both the cytosolic and the particulate fraction of the cell. Removal of the particulate fraction resulted in a 2-fold enhancement of NMT activity. Reconstitution of the particulate fraction and cytosolic fraction resulted in inhibition of the elevated cytosolic NMT activity. These results indicated the existence of putative inhibitor(s) activity of NMT located in the particulate fraction of bovine brain. The inhibitor was stable to heat and was identified as a protein, on the basis of its susceptibility to the proteases trypsin and chymotrypsin. Protease degradation first required the delipidation of the particulate fraction. The inhibitor was purified to near-homogeneity by heat treatment, solvent extraction and Sephacryl S-300 gelfiltration column chromatography. The inhibitor was purified 630-fold from the particulate fraction with a 20% yield. The protein inhibitor had an apparent molecular mass of 92 kDa by gel filtration and 71 kDa by SDS/PAGE, indicating the protein is monomeric. The inhibitor did not interact directly with myristoyl-CoA and possessed no protease, thioesterase or demyristoylase activity. Purified inhibitor protein inhibited the formation of 1167 pmol of myristoyl-peptide/min per mg of protein.
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