ArticleMolecular and cellular biology1993
Expression of the transcriptional activator LAC9 (KlGAL4) in Kluyveromyces lactis is controlled by autoregulation.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis.Microbial cell factories · 2012Article
- A kinetic model of TBP auto-regulation exhibits bistability.Biology direct · 2010Article
- Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant.Applied and environmental microbiology · 2008Article
- The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP.Molecular and cellular biology · 1999Article
- Glucose represses the lactose-galactose regulon in Kluyveromyces lactis through a SNF1 and MIG1- dependent pathway that modulates galactokinase (GAL1) gene expression.Nucleic acids research · 1997Article
- Positive autoregulation of the yeast transcription factor Pdr3p, which is involved in control of drug resistance.Molecular and cellular biology · 1995Article
- Centromere promoter factors (CPF1) of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis are functionally exchangeable, despite low overall homology.Current genetics · 1994Article
- Gal80 proteins of Kluyveromyces lactis and Saccharomyces cerevisiae are highly conserved but contribute differently to glucose repression of the galactose regulon.Molecular and cellular biology · 1993Article
- Autoregulation of GAL4 transcription is essential for rapid growth of Kluyveromyces lactis on lactose and galactose.Nucleic acids research · 1993Article
- The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression.Antonie van LeeuwenhoekReview
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2 authors at 1 institution in 1 country.
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Abstract
The concentration of the transcriptional activator LAC9 (KlGAL4) of Kluyveromyces lactis is moderately regulated by the carbon source as is the case for GAL4, its homolog in Saccharomyces cerevisiae. Expression of the LAC9 gene is induced about twofold in galactose. This induction is due to autoregulation. The LAC9 gene product binds to a low-affinity binding site in the LAC9 promoter and moderately activates transcription in response to galactose above a basal level. As for the LAC9-controlled metabolic genes, induction of LAC9 is inhibited in the presence of glucose. This inhibition of induction is a prerequisite for glucose repression of the lactose-galactose metabolic pathway. On the other hand, induced LAC9 levels are required for optimal growth on galactose, since mutating the LAC9 binding site in the LAC9 promoter resulted in poor growth and reduced expression of LAC9-controlled genes. Thus, in addition to the GAL80-dependent regulation by protein-protein interaction, the regulation of LAC9 gene expression is an important parameter in determining carbon source control of the LAC-GAL regulon. Although the mode of control is different, the pattern of LAC9 gene regulation resembles that of the S. cerevisiae GAL4 gene, being lower in glucose and glucose-galactose than in galactose.
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