ArticleMolecular and cellular biology1993
Functional analysis of a stable transcription arrest site in the first intron of the murine adenosine deaminase gene.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Sequence-Modified Antibiotic Resistance Genes Provide Sustained Plasmid-Mediated Transgene Expression in Mammals.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- TFIIS.h, a new target of p53, regulates transcription efficiency of pro-apoptotic bax gene.Scientific reports · 2016Article
- Noncanonical transcript forms in yeast and their regulation during environmental stress.RNA (New York, N.Y.) · 2010Article
- Characterization of a novel RNA polymerase II arrest site which lacks a weak 3' RNA-DNA hybrid.Nucleic acids research · 2004Article
- In vitro activity of the baculovirus late expression factor LEF-5.Journal of virology · 2002Article
- Transcription elongation factor SII.BioEssays : news and reviews in molecular, cellular and developmental biology · 2000Review
- Recognition of a human arrest site is conserved between RNA polymerase II and prokaryotic RNA polymerases.The Journal of biological chemistry · 1998Article
- Multiple rounds of transcription by RNA polymerase II at covalently cross-linked templates.Nucleic acids research · 1998Article
- Dual function of the intron of the rat insulin I gene in regulation of gene expression.Diabetologia · 1996Article
- Positive regulation of general transcription factor SIII by a tailed ubiquitin homolog.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Disruption of the adenosine deaminase (ADA) gene using a dicistronic promoterless construct: production of an ADA-deficient homozygote ES cell line.Transgenic research · 1995Article
- Sarkosyl block of transcription reinitiation by RNA polymerase II as visualized by the colliding polymerases reinitiation assay.Nucleic acids research · 1994Article
- Control of transcription arrest in intron 1 of the murine adenosine deaminase gene.Molecular and cellular biology · 1994Article
- Sp1 is essential for both enhancer-mediated and basal activation of the TATA-less human adenosine deaminase promoter.Nucleic acids research · 1994Article
- Premature termination of tubulin gene transcription in Xenopus oocytes is due to promoter-dependent disruption of elongation.Molecular and cellular biology · 1993Article
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4 authors.
Funding
Abstract
Transcription arrest plays a role in regulating the expression of a number of genes, including the murine adenosine deaminase (ADA) gene. We have previously identified two prominent arrest sites at the 5' end of the ADA gene: one in the first exon and one in the first intron (J. W. Innis and R. E. Kellems, Mol. Cell. Biol. 11:5398-5409, 1991). Here we report the functional characterization of the intron 1 arrest site, located 137 to 145 nucleotides downstream of the cap site. We have determined, using gel filtration, that the intron 1 arrest site is a stable RNA polymerase II pause site and that the transcription elongation factor SII promotes read-through at this site. Additionally, the sequence determinants for the pause are located within a 37-bp fragment encompassing this site (+123 to +158) and can direct transcription arrest in an orientation-dependent manner in the context of the ADA and adenovirus major late promoters. Specific point mutations in this region increase or decrease the relative pausing efficiency. We also show that the sequence determinants for transcription arrest can function when placed an additional 104 bp downstream of their natural position.
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