ArticleMolecular and cellular biology1993
In vitro definition of the yeast RNA polymerase I enhancer.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Development of a Virus-Based Reporter System for Functional Analysis of Plant rRNA Gene Promoter.Frontiers in microbiology · 2021Article
- Establishment of canine RNA polymerase I-driven reverse genetics for influenza A virus: its application for H5N1 vaccine production.Journal of virology · 2008Article
- Yeast RNA polymerase I enhancer is dispensable for transcription of the chromosomal rRNA gene and cell growth, and its apparent transcription enhancement from ectopic promoters requires Fob1 protein.Molecular and cellular biology · 2001Article
- In vivo binding and hierarchy of assembly of the yeast RNA polymerase I transcription factors.Molecular biology of the cell · 2001Article
- Survey and summary: transcription by RNA polymerases I and III.Nucleic acids research · 2000Review
- Transcriptional activity and chromatin structure of enhancer-deleted rRNA genes in Saccharomyces cerevisiae.Molecular and cellular biology · 1999Article
- Distal enhancer regulation by promoter derepression in topologically constrained DNA in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Molecular cloning and analysis of Schizosaccharomyces pombe Reb1p: sequence-specific recognition of two sites in the far upstream rDNA intergenic spacer.Nucleic acids research · 1997Article
- Activated levels of rRNA synthesis in fission yeast are driven by an intergenic rDNA region positioned over 2500 nucleotides upstream of the initiation site.Nucleic acids research · 1997Article
- The Xenopus RNA polymerase I transcription factor, UBF, has a role in transcriptional enhancement distinct from that at the promoter.The EMBO journal · 1997Article
- Metazoan rDNA enhancer acts by making more genes transcriptionally active.The Journal of cell biology · 1996Article
- Requirements for activity of the yeast mitotic recombination hotspot HOT1: RNA polymerase I and multiple cis-acting sequences.Genetics · 1995Article
- Transcription in the yeast rRNA gene locus: distribution of the active gene copies and chromatin structure of their flanking regulatory sequences.Molecular and cellular biology · 1995Article
- Identification of an origin of bidirectional DNA replication in the ubiquitously expressed mammalian CAD gene.Molecular and cellular biology · 1995Article
- Sarkosyl block of transcription reinitiation by RNA polymerase II as visualized by the colliding polymerases reinitiation assay.Nucleic acids research · 1994Article
- Chromatin structure and transcriptional activity around the replication forks arrested at the 3' end of the yeast rRNA genes.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In vitro conditions are reported under which an EcoRI-HpaI fragment of the Saccharomyces cerevisiae ribosomal gene spacer will enhance transcription from an adjacent RNA polymerase I promoter. Enhancement is largely independent of orientation and distance and is proportional to copy number. Mapping experiments reveal that two separate regions of the EcoRI-HpaI fragment are independently capable of promoter stimulation. These regions appear to correspond to elements which have been shown by previous workers to cause enhancement in vivo. Using the detergent Sarkosyl to limit the number of rounds of transcription from each promoter, we found that the degree of enhancement is similar whether one or many rounds of transcription occur. This finding supports a model in which the enhancer increases the number of stable promoter complexes but does not alter the loading of polymerase on an active promoter. Once the stable promoter complex is formed, the enhancer can be physically severed from the promoter with no loss of enhancement. Likewise, the upstream activation region of the promoter can be severed from the core promoter domain once the stable complex has been formed. These results are interpreted to mean that the enhancer functions only to assist stable complex formation and, once that is accomplished, the enhancer is dispensable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.