ArticleThe Journal of clinical investigation1993
Cloning of a virulence factor of Entamoeba histolytica. Pathogenic strains possess a unique cysteine proteinase gene.
Article in The Journal of clinical investigation, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 81 citations in OpenAlex.
- Extracellular Cysteine Proteases of Key Intestinal Protozoan Pathogens-Factors Linked to Virulence and Pathogenicity.International journal of molecular sciences · 2023Review
- Comparison of Proteins Secreted into Extracellular Space of Pathogenic and Non-pathogenic Acanthamoeba castellanii.The Korean journal of parasitology · 2018Article
- Proteome analysis of excretory-secretory proteins ofClinical proteomics · 2016Article
- Proteases from Entamoeba spp. and Pathogenic Free-Living Amoebae as Virulence Factors.Journal of tropical medicine · 2013Article
- Entamoeba histolytica induces intestinal cathelicidins but is resistant to cathelicidin-mediated killing.Infection and immunity · 2012Article
- Excreted/secreted proteins from trypanosome procyclic strains.Journal of biomedicine & biotechnology · 2010Article
- The cathepsin L of Toxoplasma gondii (TgCPL) and its endogenous macromolecular inhibitor, toxostatin.Molecular and biochemical parasitology · 2009Article
- Intestinal invasion by Entamoeba histolytica.Sub-cellular biochemistry · 2008Article
- Direct amplification and genotyping of Dientamoeba fragilis from human stool specimens.Journal of clinical microbiology · 2004Article
- The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation.Eukaryotic cell · 2003Article
- A surface amebic cysteine proteinase inactivates interleukin-18.Infection and immunity · 2003Article
- Entamoeba histolytica cysteine proteinases disrupt the polymeric structure of colonic mucin and alter its protective function.Infection and immunity · 2003Article
- Pathogenesis of intestinal amebiasis: from molecules to disease.Clinical microbiology reviews · 2000Review
- Cysteine proteinases and the pathogenesis of amebiasis.Clinical microbiology reviews · 2000Review
- Down regulation of Entamoeba histolytica virulence by monoxenic cultivation with Escherichia coli O55 is related to a decrease in expression of the light (35-kilodalton) subunit of the Gal/GalNAc lectin.Infection and immunity · 1999Article
- Antisense inhibition of expression of cysteine proteinases affects Entamoeba histolytica-induced formation of liver abscess in hamsters.Infection and immunity · 1999Article
- Progress towards development of a vaccine for amebiasis.Clinical microbiology reviews · 1997Review
- Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica.Antimicrobial agents and chemotherapy · 1997Article
- The invasiveness of Entamoeba histolytica - a continuing enigma.Clinical molecular pathology · 1996Article
- Contact-dependent transfer of the galactose-specific lectin of Entamoeba histolytica to the lateral surface of enterocytes in culture.Infection and immunity · 1995Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Cysteine proteinases are hypothesized to be important virulence factors of Entamoeba histolytica, the causative agent of amebic dysentery and liver abscesses. The release of a histolytic cysteine proteinase from E. histolytica correlates with the pathogenicity of both axenic strains and recent clinical isolates as determined by clinical history of invasive disease, zymodeme analysis, and cytopathic effect. We now show that pathogenic isolates have a unique cysteine proteinase gene (ACP1). Two other cysteine proteinase genes (ACP2, ACP3) are 85% identical to each other and are present in both pathogenic and nonpathogenic isolates. ACP1 is only 35 and 45% identical in sequence to the two genes found in all isolates and is present on a distinct chromosome-size DNA fragment. Presence of the ACP1 gene correlates with increased proteinase expression and activity in pathogenic isolates as well as cytopathic effect on a fibroblast monolayer, an in vitro assay of virulence. Analysis of the predicted amino acid sequence of the ACP1 proteinase gene reveals homology with cysteine proteinases released by activated macrophages and invasive cancer cells, suggesting an evolutionarily conserved mechanism of tissue invasion. The observation that a histolytic cysteine proteinase gene is present only in pathogenic isolates of E. histolytica suggests that this aspect of virulence in amebiasis is genetically predetermined.
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