ArticleProceedings of the National Academy of Sciences of the United States of America1993
Association of GAD-65, but not of GAD-67, with the Golgi complex of transfected Chinese hamster ovary cells mediated by the N-terminal region.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons.eLife · 2017Article
- Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.Molecular brain · 2015Article
- Advances in the cellular immunological pathogenesis of type 1 diabetes.Journal of cellular and molecular medicine · 2014Review
- Autoimmune stiff person syndrome and related myelopathies: understanding of electrophysiological and immunological processes.Muscle & nerve · 2012Review
- Palmitoylation and trafficking of GAD65 are impaired in a cellular model of Huntington's disease.The Biochemical journal · 2012Article
- GABA(A) receptor and glycine receptor activation by paracrine/autocrine release of endogenous agonists: more than a simple communication pathway.Molecular neurobiology · 2011Review
- A Role for GAT-1 in Presynaptic GABA Homeostasis?Frontiers in cellular neuroscience · 2011Article
- Two distinct mechanisms target GAD67 to vesicular pathways and presynaptic clusters.The Journal of cell biology · 2010Article
- Heterogeneity of the supramammillary-hippocampal pathways: evidence for a unique GABAergic neurotransmitter phenotype and regional differences.The European journal of neuroscience · 2010Article
- Rapid decrease of GAD 67 content before the convulsion induced by hyperbaric oxygen exposure.Neurochemical research · 2008Article
- Expression and function of SNAP-25 as a universal SNARE component in GABAergic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Improved in planta expression of the human islet autoantigen glutamic acid decarboxylase (GAD65).Transgenic research · 2003Article
- Demonstration of functional coupling between gamma -aminobutyric acid (GABA) synthesis and vesicular GABA transport into synaptic vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A combination of three distinct trafficking signals mediates axonal targeting and presynaptic clustering of GAD65.The Journal of cell biology · 2002Article
- Autoimmunity to beta IV spectrin in paraneoplastic lower motor neuron syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- betaIV spectrin, a new spectrin localized at axon initial segments and nodes of ranvier in the central and peripheral nervous system.The Journal of cell biology · 2000Article
- GERp95, a membrane-associated protein that belongs to a family of proteins involved in stem cell differentiation.Molecular biology of the cell · 1999Article
- Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Motifs and structural fold of the cofactor binding site of human glutamate decarboxylase.Protein science : a publication of the Protein Society · 1998Article
- GAD-reactive CD4+ Th1 cells induce diabetes in NOD/SCID mice.The Journal of clinical investigation · 1998Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Glutamic acid decarboxylase (GAD) is the enzyme responsible for synthesis of the neurotransmitter gamma-aminobutyric acid in neurons and pancreatic beta cells. It is represented by two isoforms, GAD-65 and GAD-67, which are the products of two different genes and differ substantially only at their N-terminal regions. GAD-65 is a dominant autoantigen in stiff-man syndrome and insulin-dependent diabetes mellitus. In neurons and beta cells, GAD is concentrated around synaptic vesicles and synaptic-like microvesicles, respectively, as well as in the area of the Golgi complex. The mechanisms responsible for specific targeting of GAD to these organelles are not yet understood. The elucidation of the mechanism of subcellular targeting of GAD may be relevant to understanding its role as an autoantigen. In this study, the cloned genes for GAD-65 and GAD-67 were expressed separately in Chinese hamster ovary (CHO) cells and COS cells. While GAD-67 had a diffuse cytoplasmic localization, GAD-65 had a punctate distribution, with most of the immunoreactivity being concentrated in the area of the Golgi complex. A chimeric protein in which the 88 N-terminal amino acids of GAD-67 were replaced by the 83 N-terminal amino acids of GAD-65 was targeted to the Golgi complex, indicating that the N-terminal region of GAD-65 contains a targeting signal sufficient for directing the remaining portion of the molecule, highly similar in GAD-65 and GAD-67, to the Golgi complex-associated structures.
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