ArticleProceedings of the National Academy of Sciences of the United States of America1993
An 83-nucleotide promoter of the acetylcholine receptor epsilon-subunit gene confers preferential synaptic expression in mouse muscle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 55 citations in OpenAlex.
- The Structure, Function, and Physiology of the Fetal and Adult Acetylcholine Receptor in Muscle.Frontiers in molecular neuroscience · 2020Review
- Article
- Postsynaptic chromatin is under neural control at the neuromuscular junction.The EMBO journal · 2007Article
- Phosphorylation-elicited quaternary changes of GA binding protein in transcriptional activation.Molecular and cellular biology · 2003Article
- Aberrant development of motor axons and neuromuscular synapses in MyoD-null mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- A novel pathway for MuSK to induce key genes in neuromuscular synapse formation.The Journal of cell biology · 2003Article
- Muscle-regulated expression and determinants for neuromuscular junctional localization of the mouse RIalpha regulatory subunit of cAMP-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- The Ets transcription factor GABP is required for postsynaptic differentiation in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Essential roles of c-JUN and c-JUN N-terminal kinase (JNK) in neuregulin-increased expression of the acetylcholine receptor epsilon-subunit.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- An intronic enhancer containing an N-box motif is required for synapse- and tissue-specific expression of the acetylcholinesterase gene in skeletal muscle fibers.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Induction of utrophin gene expression by heregulin in skeletal muscle cells: role of the N-box motif and GA binding protein.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Synapse-specific and neuregulin-induced transcription require an ets site that binds GABPalpha/GABPbeta.Genes & development · 1998Article
- Implication of a multisubunit Ets-related transcription factor in synaptic expression of the nicotinic acetylcholine receptor.The EMBO journal · 1998Article
- Identification of a neuregulin and protein-tyrosine phosphatase response element in the nicotinic acetylcholine receptor epsilon subunit gene: regulatory role of an Rts transcription factor.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Myasthenia gravis as a prototype autoimmune receptor disease.Immunologic research · 1997Review
- Accumulation in fetal muscle and localization to the neuromuscular junction of cAMP-dependent protein kinase A regulatory and catalytic subunits RI alpha and C alpha.The Journal of cell biology · 1996Article
- Substrate-bound agrin induces expression of acetylcholine receptor epsilon-subunit gene in cultured mammalian muscle cells.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Identification of a DNA element determining synaptic expression of the mouse acetylcholine receptor delta-subunit gene.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Neural regulation of acetylcholinesterase mRNAs at mammalian neuromuscular synapses.The Journal of cell biology · 1994Article
- Neural regulation of muscle acetylcholine receptor epsilon- and alpha-subunit gene promoters in transgenic mice.The Journal of cell biology · 1993Article
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3 authors at 1 institution in 1 country.
Funding
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Abstract
The expression of the acetylcholine receptor epsilon-subunit gene is restricted to the endplate of adult muscle fibers. We have started to study the regulatory elements of the epsilon-subunit gene promoter that are important for its synaptic expression. We used, for this purpose, a rapid method of in vivo expression after DNA injection into the muscle tissue [Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A. & Felgner, P. L. (1990) Science 247, 1465-1468]. Our results show that a construction containing 83 nucleotides upstream from the transcription start site is sufficient to obtain preferential endplate expression. Moreover, mutation of a MyoD binding site located around position-70 does not alter this synaptic expression. We also studied the expression of this promoter in vitro in muscle primary cultures and showed the presence of a positive element between positions -122 and -83. Comparison of in vivo and in vitro results reveals that the elements important for in vivo localization at the synapse and in vitro expression in cultured muscle cells may differ.
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