ArticleProceedings of the National Academy of Sciences of the United States of America1993
In vitro assembly of multiprotein complexes containing alpha, beta, and gamma tubulin, heat shock protein HSP70, and elongation factor 1 alpha.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 79 citations in OpenAlex.
- Different functions of HOPS isoforms in the cell: HOPS shuttling isoform is determined by RIP cleavage system.Cell cycle (Georgetown, Tex.) · 2014Article
- Article
- Hsp70 and thermal pretreatment mitigate developmental damage caused by mitotic poisons in Drosophila.Cell stress & chaperones · 2002Article
- Unbalanced expression of the different subunits of elongation factor 1 in diabetic skeletal muscle.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Different Sp1 family members differentially affect transcription from the human elongation factor 1 A-1 gene promoter.The Biochemical journal · 1998Article
- Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of alpha/beta tubulin.The Journal of cell biology · 1997Article
- Role of tissue specific alternative pre-mRNA splicing in the differentiation of the erythrocyte membrane.Transactions of the American Clinical and Climatological Association · 1997Review
- Molecular cloning, characterization and expression of an elongation factor 1 alpha gene in maize.Plant molecular biology · 1995Article
- gamma-tubulin is a minus end-specific microtubule binding protein.The Journal of cell biology · 1995Article
- Gamma-tubulin can both nucleate microtubule assembly and self-assemble into novel tubular structures in mammalian cells.The Journal of cell biology · 1995Article
- Identification of the human prostatic carcinoma oncogene PTI-1 by rapid expression cloning and differential RNA display.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Function of the p86 subunit of eukaryotic initiation factor (iso)4F as a microtubule-associated protein in plant cells.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Convergent and parallel activation of low-conductance potassium channels by calcium and cAMP-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Role of 300 kDa complexes as intermediates in tubulin folding and dimerization: characterization of a 25 kDa cytosolic protein involved in the GTP-dependent release of monomeric tubulin.The Biochemical journal · 1994Article
- A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha.The Plant cell · 1994Article
- Protein synthesis elongation factor EF-1 alpha expression and longevity in Drosophila melanogaster.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Biochemical dissection of AP-1 recruitment onto Golgi membranes.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated two sets of multiprotein complexes from supernatants from high-speed centrifugation of nocodazole-arrested CHO cells. One set, assembled in vitro after a 37 degrees C incubation in the presence of ATP or GTP, is composed of equivalent amounts of alpha- and beta-tubulin and a 50-kDa protein, provisionally identified as elongation factor 1 alpha. These complexes, which are heterogeneous in size when analyzed by sucrose gradient ultracentrifugation, also contain the cognate form of heat shock protein HSP70 and gamma-tubulin, a tubulin isoform of low abundance, along with other proteins known to be involved in the regulation of mitosis. Similar but distinct complexes assemble in vitro if the same extracts are incubated at 37 degrees C without added nucleotides; multiprotein complexes generated under these conditions lack HSP70 but contain instead a 43-kDa protein identified as an actin isoform. Both sets of assembled complexes exhibit a globular substructure when analyzed by electron microscopy, and their size distribution suggests that they assemble by the step-wise addition of smaller precursors. The properties of these multiprotein complexes and their presence in cells arrested in a stage between prophase and metaphase suggest that they may be precursors to mitotic centrosomes and are possibly involved in mitotic spindle nucleation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.