ArticleProceedings of the National Academy of Sciences of the United States of America1993
Antibody-promoted dimerization bypasses the regulation of DNA binding by the heme domain of the yeast transcriptional activator HAP1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Mitochondrial-nuclear heme trafficking in budding yeast is regulated by GTPases that control mitochondrial dynamics and ER contact sites.Journal of cell science · 2020Article
- Heme bioavailability and signaling in response to stress in yeast cells.The Journal of biological chemistry · 2018Article
- The Q-System: A Versatile Expression System for Drosophila.Methods in molecular biology (Clifton, N.J.) · 2016Review
- Improved and expanded Q-system reagents for genetic manipulations.Nature methods · 2015Article
- The heme activator protein Hap1 represses transcription by a heme-independent mechanism in Saccharomyces cerevisiae.Genetics · 2005Article
- Structure of HAP1-PC7 bound to DNA: implications for DNA recognition and allosteric effects of DNA-binding on transcriptional activation.Nucleic acids research · 2000Article
- A new class of repression modules is critical for heme regulation of the yeast transcriptional activator Hap1.Molecular and cellular biology · 1999Article
- Molecular mechanism governing heme signaling in yeast: a higher-order complex mediates heme regulation of the transcriptional activator HAP1.Molecular and cellular biology · 1998Article
- FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences.The EMBO journal · 1998Article
- Comparative amino acid sequence analysis of the C6 zinc cluster family of transcriptional regulators.Nucleic acids research · 1996Article
- A novel DNA binding motif for yeast zinc cluster proteins: the Leu3p and Pdr3p transcriptional activators recognize everted repeats.Molecular and cellular biology · 1996Article
- The C6 zinc cluster dictates asymmetric binding by HAP1.The EMBO journal · 1996Article
- Heme binding by a bacterial repressor protein, the gene product of the ferric uptake regulation (fur) gene of Escherichia coli.Journal of protein chemistry · 1996Article
- Mutations in target DNA elements of yeast HAP1 modulate its transcriptional activity without affecting DNA binding.Nucleic acids research · 1996Article
- Multiple domains mediate heme control of the yeast activator HAP1.Molecular & general genetics : MGG · 1995Article
- A novel allele of HAP1 causes uninducible expression of HEM13 in Saccharomyces cerevisiae.Genetics · 1994Article
- Evidence that TUP1/SSN6 has a positive effect on the activity of the yeast activator HAP1.Genetics · 1994Article
- Compilation of sequence-specific DNA-binding proteins implicated in transcriptional control in fungi.Nucleic acids research · 1993Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The yeast transcriptional activator HAP1 contains a DNA-binding domain homologous to the zinc finger of GAL4 and an adjacent regulatory domain that blocks DNA binding in the absence of the inducer heme. We show that short HAP1 fragments containing the zinc finger are unable to bind to DNA but can be rescued by antibody to the HAP1 zinc finger. These fragments are missing a coiled-coil sequence similar to that within the dimerization domain of GAL4 and dimerization domains of myosin heavy chain. We surmise that the antibody promotes DNA binding by bringing together two monomers. Interestingly, the antibody will also promote DNA binding of a larger HAP1 fragment containing the DNA-binding and the heme-regulatory domains. This suggests that the regulatory domain acts by preventing dimerization of HAP1 in the absence of heme. Consistent with this view is an in vivo assay that also reveals that heme promotes HAP1 dimerization in yeast cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.