ArticleProceedings of the National Academy of Sciences of the United States of America1993
Adenine nucleoside diphosphates block adaptation of mechanoelectrical transduction in hair cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 66 citations in OpenAlex.
- Cy3-ATP labeling of unfixed, permeabilized mouse hair cells.Scientific reports · 2021Article
- Chemomechanical regulation of myosin Ic cross-bridges: Deducing the elastic properties of an ensemble from single-molecule mechanisms.PLoS computational biology · 2017Article
- Article
- Integrating the biophysical and molecular mechanisms of auditory hair cell mechanotransduction.Nature communications · 2011Review
- Review
- Adenylate kinase and AMP signaling networks: metabolic monitoring, signal communication and body energy sensing.International journal of molecular sciences · 2009Review
- Review
- Article
- The micromachinery of mechanotransduction in hair cells.Annual review of neuroscience · 2007Review
- Article
- Myosin I and adaptation of mechanical transduction by the inner ear.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Two adaptation processes in auditory hair cells together can provide an active amplifier.Biophysical journal · 2003Article
- Putting ion channels to work: mechanoelectrical transduction, adaptation, and amplification by hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Two mechanisms for transducer adaptation in vertebrate hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Plasma membrane Ca2+-ATPase extrudes Ca2+ from hair cell stereocilia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Hair cell-specific splicing of mRNA for the alpha1D subunit of voltage-gated Ca2+ channels in the chicken's cochlea.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Myosin VIIA is required for aminoglycoside accumulation in cochlear hair cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- ATPase activity of myosin in hair bundles of the bullfrog's sacculus.Biophysical journal · 1997Article
- Rapid, active hair bundle movements in hair cells from the bullfrog's sacculus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Calmodulin controls adaptation of mechanoelectrical transduction by hair cells of the bullfrog's sacculus.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
By adapting to sustained stimuli, hair cells in the internal ear retain their sensitivity to minute transient displacements. Because one model for adaptation asserts that this process is mediated by a myosin isozyme, we reasoned that we should be able to arrest adaptation by interfering with myosin's ATPase cycle though introduction of ADP into hair cells. During tight-seal, whole-cell recordings of transduction currents in cells isolated from bullfrog (Rana catesbeiana) sacculus, dialysis with 5-25 mM ADP gave variable results. In half of the cells examined, the rate of adaptation remained unchanged or even increased; adaptation was blocked in the remaining cells. Because we suspected that the variable effect of ADP resulted from the conversion of ADP to ATP by adenylate kinase, we employed the ADP analog adenosine 5'-[beta-thio]diphosphate (ADP[beta S]), which is not a substrate for adenylate kinase. Adaptation consistently disappeared in the presence of 1-10 mM ADP[beta S]; in addition, the transduction channels' open probability at rest grew from approximately 0.1 to 0.8 or more. Both effects could be reversed by 2 mM ATP. When used in conjunction with the adenylate kinase inhibitor P1,P5-bis(5'-adenosyl) pentaphosphate (Ap5A), ADP had effects similar to those of ADP[beta S]. These results suggest that adaptation by hair cells involves adenine nucleotides, and they lend support to the hypothesis that the adaptation process is powered by a myosin motor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.