ArticleNucleic acids research1993
Tissue-specific and developmentally regulated alternative splicing of a visceral isoform of smooth muscle myosin heavy chain.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 83 citations in OpenAlex.
- MBNL loss of function in smooth muscle as a model for myotonic dystrophy associated gastrointestinal dysmotility.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Loss of smooth muscle myosin heavy chain results in the bladder and stomach developing lesion during foetal development in mice.Journal of genetics · 2018Article
- Developmentally regulated alternative splicing is perturbed in type 1 diabetic skeletal muscle.Muscle & nerve · 2017Article
- Unphosphorylated calponin enhances the binding force of unphosphorylated myosin to actin.Biochimica et biophysica acta · 2013Article
- Alterations in the contractile phenotype of the bladder: lessons for understanding physiological and pathological remodelling of smooth muscle.Journal of cellular and molecular medicine · 2012Review
- Myosin, transgelin, and myosin light chain kinase: expression and function in asthma.American journal of respiratory and critical care medicine · 2009Article
- Tuning smooth muscle contraction by molecular motors.Journal of molecular medicine (Berlin, Germany) · 2003Review
- Alterations in expression of myosin and myosin light chain kinases in response to vascular injury.American journal of physiology. Cell physiology · 2000Article
- Variable surface loops and myosin activity: accessories to a motor.Journal of muscle research and cell motility · 2000Review
- A 7-amino-acid insert in the heavy chain nucleotide binding loop alters the kinetics of smooth muscle myosin in the laser trap.Journal of muscle research and cell motility · 1998Article
- A myosin family reunion.Journal of muscle research and cell motility · 1996Review
- Characterization of myosin II isoforms containing insertions of amino acids in the flexible loop near the ATP-binding pocket.Biophysical journal · 1995Article
- Scallop striated and smooth muscle myosin heavy-chain isoforms are produced by alternative RNA splicing from a single gene.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Myosin isoforms in smooth muscle: how may they affect function and structure?Journal of muscle research and cell motility · 1993Review
- Flash photolysis studies of relaxation and cross-bridge detachment: higher sensitivity of tonic than phasic smooth muscle to MgADP.Journal of muscle research and cell motility · 1993Article
- Biosynthesis of nitric oxide activates iron regulatory factor in macrophages.The EMBO journal · 1993Article
- Expression of myosin isoforms in the smooth muscle of human corpus cavernosum.International journal of impotence researchArticle
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous work demonstrated that the rabbit smooth muscle myosin heavy chain gene showed sequence divergence at the 25kDa/50kDa junction of the S1 subfragment when compared to chicken gizzard and chicken epithelial nonmuscle myosin. RNase protection analysis with a probe spanning this region detected two partially protected fragments which were not present in RNA from vascular tissue and only found in RNA from visceral tissue. The polymerase chain reaction was used to amplify a 162bp product from primers spanning the putative region of divergence and DNA sequence analysis revealed a seven amino acid insertion not previously detected in other characterised cDNA clones. RNase protection analysis using the PCR product as probe showed that the inserted sequence was expressed exclusively in RNA from visceral tissue. Similar RNA analysis showed that the visceral isoform was not expressed in 20 day fetal rabbit smooth muscle tissues. These results indicated that the new visceral isoform was expressed in a tissue-specific and developmentally regulated manner. Genomic DNA sequencing and mapping of the exon-intron boundaries showed that the visceral isoform was the product of cassette-type alternative splicing. The inclusion of a visceral-specific sequence near the Mg-ATPase domain and at the 25kDa/50kDa junction suggests that the visceral isoform may be important for myosin function in smooth muscle cells.
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