ArticleProceedings of the National Academy of Sciences of the United States of America1993
Charge-reversion mutagenesis of Dictyostelium actin to map the surface recognized by myosin during ATP-driven sliding motion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 77 citations in OpenAlex.
- Integrated structural model of the palladin-actin complex using XL-MS, docking, NMR, and SAXS.Protein science : a publication of the Protein Society · 2025Article
- Article
- Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments.Nature structural & molecular biology · 2014Article
- Functional characterization of the human α-cardiac actin mutations Y166C and M305L involved in hypertrophic cardiomyopathy.Cellular and molecular life sciences : CMLS · 2012Article
- Functional adaptation between yeast actin and its cognate myosin motors.The Journal of biological chemistry · 2011Article
- Unidirectional Brownian motion observed in an in silico single molecule experiment of an actomyosin motor.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Dominant negative mutant actins identified in flightless Drosophila can be classified into three classes.The Journal of biological chemistry · 2010Article
- Myosin loop 2 is involved in the formation of a trimeric complex of twitchin, actin, and myosin.The Journal of biological chemistry · 2009Article
- Close proximity of myosin loop 3 to troponin determined by triangulation of resonance energy transfer distance measurements.Biochemistry · 2009Article
- Unphosphorylated twitchin forms a complex with actin and myosin that may contribute to tension maintenance in catch.The Journal of experimental biology · 2007Article
- A closer look at energy transduction in muscle.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Role of the N-terminal negative charges of actin in force generation and cross-bridge kinetics in reconstituted bovine cardiac muscle fibres.The Journal of physiology · 2005Article
- Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor.Biophysical journal · 2001Article
- A heterozygous mutation of beta-actin associated with neutrophil dysfunction and recurrent infection.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Adducin is an in vivo substrate for protein kinase C: phosphorylation in the MARCKS-related domain inhibits activity in promoting spectrin-actin complexes and occurs in many cells, including dendritic spines of neurons.The Journal of cell biology · 1998Article
- Characterization of mutant myosins of Dictyostelium discoideum equivalent to human familial hypertrophic cardiomyopathy mutants. Molecular force level of mutant myosins may have a prognostic implication.The Journal of clinical investigation · 1997Article
- ATPase activity of myosin in hair bundles of the bullfrog's sacculus.Biophysical journal · 1997Article
- Small segmental rearrangements in the myosin head can explain force generation in muscle.Biophysical journal · 1996Article
- Multiple- and single-molecule analysis of the actomyosin motor by nanometer-piconewton manipulation with a microneedle: unitary steps and forces.Biophysical journal · 1996Article
- Organelle-cytoskeletal interactions: actin mutations inhibit meiosis-dependent mitochondrial rearrangement in the budding yeast Saccharomyces cerevisiae.Molecular biology of the cell · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amino acid residues D24/D25, E99/E100, E360/E361, and D363/E364 in subdomain 1 of Dictyostelium actin were replaced with histidine residues by site-directed mutagenesis. Mutant actins were expressed in Dictyostelium cells and purified to homogeneity. The sliding movement of mutant actin filaments on heavy meromyosin attached to a glass surface was measured to assess the effect of the mutation on the motility of actin. For two C-terminal mutants, force generated by a single actin filament and myosin was also measured. These measurements indicated that both D24/D25 and E99/E100 are involved in ATP-driven sliding, whereas E360/E361/D363/E364 are not essential for ATP-driven sliding and force generation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.