ArticleThe Journal of cell biology1993
Noncoding regions of the gamma-actin gene influence the impact of the gene on myoblast morphology.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Nucleotide- and Protein-Dependent Functions ofMolecular biology of the cell · 2022Article
- Article
- Regulation of actin isoforms in cellular and developmental processes.Seminars in cell & developmental biology · 2020Review
- RRAD suppresses the Warburg effect by downregulating ACTG1 in hepatocellular carcinoma.OncoTargets and therapy · 2019Article
- The makings of the 'actin code': regulation of actin's biological function at the amino acid and nucleotide level.Journal of cell science · 2018Review
- Impaired muscle relaxation and mitochondrial fission associated with genetic ablation of cytoplasmic actin isoforms.The FEBS journal · 2018Article
- Article
- A novel actin mRNA splice variant regulates ACTG1 expression.PLoS genetics · 2013Article
- Isoform specificity in the relationship of actin to dendritic spines.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- beta-Actin messenger RNA localization and protein synthesis augment cell motility.The Journal of cell biology · 1997Article
- Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles.The Journal of cell biology · 1996Article
- Evolution of actin gene families of sea urchins.Journal of molecular evolution · 1994Article
- Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype.The Journal of cell biology · 1994Article
- Beta and gamma actin mRNAs are differentially located within myoblasts.The Journal of cell biology · 1993Article
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2 authors.
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Abstract
We have addressed the question of whether two highly conserved noncoding regions of the gamma-actin gene are of functional importance. Human gamma-actin gene constructs deleted for either the entire 3' untranslated region (UTR) and 3' flank or intron III sequences were transfected into mouse myoblasts and the resulting clones were analyzed for cell morphology and actin protein expression. Transfectants carrying the gamma-actin gene deleted for the 3' end (gamma 22) exhibited numerous long pseudopods and increased surface area. In contrast, transfectants expressing the gamma-actin gene deleted for intron III (gamma 156) were rounded with blebs over the cell surface and showed decreased surface area. The relative expression of beta- to gamma-actin protein decreased for both transfectant types. The total actin protein levels remained constant for the gamma 22 cells but decreased for the gamma 156 cells. The results indicate that alterations to transfectant cell morphology can be influenced by the presence or absence of different noncoding regions in the transfected gamma-actin gene. The mechanisms by which noncoding regions of the gamma-actin gene influence the impact of the gene are unknown. Nevertheless, these noncoding regions are isoform specific and highly conserved in evolution. We propose that the functional significance of the different actin isoforms may involve the properties of these noncoding regions in addition to the differences in protein sequence.
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