ArticleThe Journal of cell biology1993
Functional activation of plasma membrane anion exchangers occurs in a pre-Golgi compartment.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 92 citations in OpenAlex.
- SLC4A2 anion exchanger promotes tumour cell malignancy via enhancing net acid efflux across golgi membranes.Cellular and molecular life sciences : CMLS · 2021Article
- Human Corneal Expression of SLC4A11, a Gene Mutated in Endothelial Corneal Dystrophies.Scientific reports · 2019Article
- Stromal uptake and transmission of acid is a pathway for venting cancer cell-generated acid.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The cytoplasmic domain is essential for transport function of the integral membrane transport protein SLC4A11.American journal of physiology. Cell physiology · 2016Article
- Transmembrane water-flux through SLC4A11: a route defective in genetic corneal diseases.Human molecular genetics · 2013Article
- Antibodies against the cardiac sodium/bicarbonate co-transporter (NBCe1) as pharmacological tools.British journal of pharmacology · 2011Article
- Cytosolic H+ microdomain developed around AE1 during AE1-mediated Cl-/HCO3- exchange.The Journal of physiology · 2011Article
- Sensors and regulators of intracellular pH.Nature reviews. Molecular cell biology · 2010Review
- Bicarbonate homeostasis in excitable tissues: role of AE3 Cl-/HCO3- exchanger and carbonic anhydrase XIV interaction.American journal of physiology. Cell physiology · 2009Article
- Red fluorescent protein pH biosensor to detect concentrative nucleoside transport.The Journal of biological chemistry · 2009Article
- Article
- Slc26a6: a cardiac chloride-hydroxyl exchanger and predominant chloride-bicarbonate exchanger of the mouse heart.The Journal of physiology · 2004Article
- Transport activity of chimaeric AE2-AE3 chloride/bicarbonate anion exchange proteins.The Biochemical journal · 2003Article
- Processing of N-linked oligosaccharide depends on its location in the anion exchanger, AE1, membrane glycoprotein.The Biochemical journal · 2000Article
- Transport activity of AE3 chloride/bicarbonate anion-exchange proteins and their regulation by intracellular pH.The Biochemical journal · 1999Article
- Transmembrane folding of the human erythrocyte anion exchanger (AE1, Band 3) determined by scanning and insertional N-glycosylation mutagenesis.The Biochemical journal · 1999Article
- Possible role of anion exchanger AE2 as the intestinal monocarboxylic acid/anion antiporter.Pharmaceutical research · 1998Article
- Expression of bacteriorhodopsin in Sf9 and COS-1 cells.Journal of bioenergetics and biomembranes · 1997Article
- Article
- Functional cell surface expression of the anion transport domain of human red cell band 3 (AE1) in the yeast Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Folding and oligomerization of most plasma membrane glycoproteins, including those involved in ion transport, occur in the ER and are frequently required for their exit from this organelle. It is currently unknown, however, where or when in the biosynthetic pathway these proteins become functionally active. AE1 and AE2 are tissue-specific, plasma membrane anion transport proteins. Transient expression of AE2 in a eukaryotic cell line leads to an increase in stilbene inhibitable whole cell 35SO4(2-)-efflux consistent with its function as a plasma membrane anion exchanger. No such increased transport activity was observed in AE1 transfectants, despite the fact that the two proteins were synthesized in roughly equal portions. In contrast, both AE1 and AE2 expression resulted in significant increase in Cl-/SO4(2-)-exchange in crude microsomes demonstrating that both AE1 and AE2 cDNAs encode functional proteins. Immunofluorescence staining and pulse-chase labeling experiments revealed that while 60% of AE2 is processed to the cell surface of transfectants, AE1 is restricted to an intracellular compartment and never acquires mature oligosaccharides. Crude microsomes from transfected cells were fractionated into plasma membrane and ER-derived vesicles by con A affinity chromatography. All of the AE1 and approximately half of the cellular AE2 was eluted with the ER vesicles, confirming their intracellular localization. Anion transport measurements on these fractions confirmed that the ER-restricted anion exchangers were functional. We conclude that AE1 and AE2 acquire the ability to mediate anion exchange at an early stage of their biosynthesis, before their exit from the ER.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.