ArticleThe Journal of cell biology1993
COOH-terminal signals mediate the trafficking of a peptide processing enzyme in endocrine cells.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 80 citations in OpenAlex.
- Inside the Insulin Secretory Granule.Metabolites · 2021Review
- An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles.Nature communications · 2020Article
- A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.Journal of cellular physiology · 2019Article
- Microvillar and ciliary defects in zebrafish lacking an actin-binding bioactive peptide amidating enzyme.Scientific reports · 2018Article
- The endocytic pathways of a secretory granule membrane protein in HEK293 cells: PAM and EGF traverse a dynamic multivesicular body network together.European journal of cell biology · 2017Article
- Article
- 60 YEARS OF POMC: From POMC and α-MSH to PAM, molecular oxygen, copper, and vitamin C.Journal of molecular endocrinology · 2016Review
- Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity.Journal of cell science · 2016Article
- A histidine-rich linker region in peptidylglycine α-amidating monooxygenase has the properties of a pH sensor.The Journal of biological chemistry · 2014Article
- Role of adaptor proteins in secretory granule biogenesis and maturation.Frontiers in endocrinology · 2013Article
- Secretory granule membrane protein recycles through multivesicular bodies.Traffic (Copenhagen, Denmark) · 2010Article
- Secretory granule to the nucleus: role of a multiply phosphorylated intrinsically unstructured domain.The Journal of biological chemistry · 2009Article
- Role of cytoplasmic domain serines in intracellular trafficking of furin.Molecular biology of the cell · 2004Article
- Signaling mediated by the cytosolic domain of peptidylglycine alpha-amidating monooxygenase.Molecular biology of the cell · 2001Article
- Chimeric forms of furin and TGN38 are transported with the plasma membrane in the trans-Golgi network via distinct endosomal pathways.The Journal of cell biology · 1999Article
- Induction of integral membrane PAM expression in AtT-20 cells alters the storage and trafficking of POMC and PC1.The Journal of cell biology · 1999Article
- The pro region is not required for the expression or intracellular routeing of carboxypeptidase E.The Biochemical journal · 1997Article
- Targeting of P-selectin to two regulated secretory organelles in PC12 cells.The Journal of cell biology · 1996Article
- Inositol 1,4,5-trisphosphate receptors in endocrine cells: localization and association in hetero- and homotetramers.Molecular biology of the cell · 1996Article
- Human CAP18: a novel antimicrobial lipopolysaccharide-binding protein.Infection and immunity · 1995Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Peptidylglycine alpha-amidating monooxygenase (PAM) catalyzes the COOH-terminal amidation of bioactive peptides through a two step reaction catalyzed by separate enzymes contained within the PAM precursor. To characterize the trafficking of integral membrane PAM proteins in neuroendocrine cells, we have generated stable AtT-20 cell lines expressing full length and COOH-terminally truncated integral membrane PAM proteins. Full length integral membrane PAM was present on the cell surface in low but detectable amounts and PAM proteins which reached the cell surface were rapidly internalized but not immediately degraded in lysosomes. Internalized PAM complexed with PAM antibody was found in a subcellular compartment which overlapped with internalized transferrin and with structures binding WGA. Thus the punctate juxtanuclear staining of full length PAM represents PAM in endosomes. Endoproteolytic processing of full length PAM-1 and PAM-2 resulted in the secretion of soluble PAM proteins; the secretion of these soluble PAM proteins was stimulus dependent. Although some of the truncated PAM protein was also processed and stored in AtT-20 cells, much of the expressed protein was redistributed to the plasma membrane. Soluble proteins not observed in large amounts in cells expressing full length PAM were released from the surface of cells expressing truncated PAM and little internalization of truncated integral membrane PAM was observed. Thus, the COOH-terminal domain of PAM contains information important for its trafficking within the regulated secretory pathway as well as information necessary for its retrieval from the cell surface.
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