ArticleThe Journal of cell biology1993
Affinity modulation of integrin alpha 5 beta 1: regulation of the functional response by soluble fibronectin.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 249 citations in OpenAlex.
- The integrin odyssey - a journey full of fundamental discoveries.Journal of cell science · 2025Article
- Fibronectin fragments generated by pancreatic trypsin act as endogenous inhibitors of pancreatic tumor growth.Journal of experimental & clinical cancer research : CR · 2023Article
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- Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated αFrontiers in cell and developmental biology · 2019Article
- Extracellular Protein Fibulin-7 and Its C-Terminal Fragment Have In Vivo Antiangiogenic Activity.Scientific reports · 2018Article
- RARα/RXR synergism potentiates retinoid responsiveness in cutaneous T-cell lymphoma cell lines.Experimental dermatology · 2017Article
- Conformational equilibria and intrinsic affinities define integrin activation.The EMBO journal · 2017Article
- Retinoic acid receptor gamma impacts cellular adhesion, Alpha5Beta1 integrin expression and proliferation in K562 cells.PloS one · 2017Article
- The G protein-coupled estrogen receptor-1, GPER-1, promotes fibrillogenesis via a Shc-dependent pathway resulting in anchorage-independent growth.Hormones & cancer · 2014Article
- Multiple Effects of a Novel Epothilone Analog on Cellular Processes and Signaling Pathways Regulated by Rac1 GTPase in the Human Breast Cancer Cells.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2014Article
- 9-cis-retinoic Acid and troglitazone impacts cellular adhesion, proliferation, and integrin expression in K562 cells.PloS one · 2014Article
- 9-cis-retinoic acid promotes cell adhesion through integrin dependent and independent mechanisms across immune lineages.The Journal of nutritional biochemistry · 2013Article
- A spatial model for integrin clustering as a result of feedback between integrin activation and integrin binding.Biophysical journal · 2012Article
- A computational analysis of the dynamic roles of talin, Dok1, and PIPKI for integrin activation.PloS one · 2011Article
- Focal adhesion kinase modulates activation of NF-kappaB by flow in endothelial cells.American journal of physiology. Cell physiology · 2009Article
- Article
- Coordinate regulation of estrogen-mediated fibronectin matrix assembly and epidermal growth factor receptor transactivation by the G protein-coupled receptor, GPR30.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Migfilin, a molecular switch in regulation of integrin activation.The Journal of biological chemistry · 2009Article
- Myocilin promotes substrate adhesion, spreading and formation of focal contacts in podocytes and mesangial cells.Histochemistry and cell biology · 2009Article
- Integrin-mediated adhesion regulates membrane order.The Journal of cell biology · 2006Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We report that a beta 1 integrin (alpha 5 beta 1) can exist in different affinity states for its soluble ligand, fibronectin. The alpha 5 beta 1 expressed by the erythroleukemic cell line K562 binds soluble fibronectin with low affinity (Kd > 1 microM), but is induced to bind it with 20-fold higher affinity (Kd-54 nM) in the presence of the anti-beta 1 mAb 8A2. This activation seems to be due to direct antibody-induced change in the receptor that does not require intracellular signaling, and is a plausible basis for the 8A2-induced enhancement of beta 1-dependent adhesion to fibronectin and other immobilized ligands (Kovach, N. L., T. M. Carlos, E. Yee, and J. M. Harlan. 1992. J. Cell Biol. 116: 499-509). Fab fragments of 8A2 bind with higher affinity to alpha 5 beta 1 receptor that is occupied by the GRG-DSP peptide ligand suggesting that the antibody functions by stabilizing a high affinity (occupied) conformer of the receptor. A functional consequence of the affinity modulation is that soluble fibronectin (at physiological concentrations) occupies the high affinity receptors, and so becomes an effective inhibitor of adhesion to immobilized fibronectin. In contrast, the majority of low affinity receptors remain unoccupied and are still to mediate cellular adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.