ArticleThe EMBO journal1993
Conversion of a trans-spliced C. elegans gene into a conventional gene by introduction of a splice donor site.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- Article
- Trans-splicing and operons in C. elegans.WormBook : the online review of C. elegans biology · 2012Review
- Cross-validated methods for promoter/transcription start site mapping in SL trans-spliced genes, established using the Ciona intestinalis troponin I gene.Nucleic acids research · 2011Article
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- Polycistronic pre-mRNA processing in vitro: snRNP and pre-mRNA role reversal in trans-splicing.Genes & development · 2010Article
- High-throughput sequence analysis of Ciona intestinalis SL trans-spliced mRNAs: alternative expression modes and gene function correlates.Genome research · 2010Article
- Functional redundancy of worm spliceosomal proteins U1A and U2B''.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Genome annotation by high-throughput 5' RNA end determination.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.Eukaryotic cell · 2003Review
- An uncapped RNA suggests a model for Caenorhabditis elegans polycistronic pre-mRNA processing.RNA (New York, N.Y.) · 2003Article
- A complex containing CstF-64 and the SL2 snRNP connects mRNA 3' end formation and trans-splicing in C. elegans operons.Genes & development · 2001Article
- mRNA 5'-leader trans-splicing in the chordates.Genes & development · 2001Article
- Intercistronic region required for polycistronic pre-mRNA processing in Caenorhabditis elegans.Molecular and cellular biology · 2001Article
- trans splicing of polycistronic Caenorhabditis elegans pre-mRNAs: analysis of the SL2 RNA.Molecular and cellular biology · 2000Article
- A new twist in trypanosome RNA metabolism: cis-splicing of pre-mRNA.RNA (New York, N.Y.) · 2000Article
- U2AF35 is encoded by an essential gene clustered in an operon with RRM/cyclophilin in Caenorhabditis elegans.RNA (New York, N.Y.) · 1999Article
- Natural trans-splicing in carnitine octanoyltransferase pre-mRNAs in rat liver.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Operons and SL2 trans-splicing exist in nematodes outside the genus Caenorhabditis.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Intronic and exonic sequences modulate 5' splice site selection in plant nuclei.Nucleic acids research · 1997Article
- Trans-splicing and alternative-tandem-cis-splicing: two ways by which mammalian cells generate a truncated SV40 T-antigen.Nucleic acids research · 1996Article
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3 authors.
Funding
Abstract
In Caenorhabditis elegans, pre-mRNAs that are trans-spliced are distinguished by the presence of an 'outron', intron-like RNA at the 5' end followed by a splice acceptor. We report that trans-splicing of the rol-6 gene can be completely suppressed simply by introducing a donor site into its 173 nt outron, at a site 50 nt upstream of the trans-splice site, thereby converting rol-6 into a conventional gene with a spliced intron near its 5' end. When the consensus donor site was inserted at sites further upstream it was less effective in replacing transplicing with cis-splicing. Surprisingly, the length of the intron was not the important variable, since lengthening of the 50 nt intron to 250 nt did not restore trans-splicing. Apparently the context into which the splice site was introduced determined the efficiency of its use. These results support the conclusion that the sole signal for trans-splicing is the presence of an outron. Clearly, cis- and trans-splice acceptor sites are interchangeable, allowing the possibility of competition between the two types of splicing.
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