ArticleMolecular and cellular biology1993
Replication-competent retroviral vectors encoding alkaline phosphatase reveal spatial restriction of viral gene expression/transduction in the chick embryo.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Development of a heat-stable alkaline phosphatase reporter system for cis-regulatory analysis and its application to 3D digital imaging of Xenopus embryonic tissues.Development, growth & differentiation · 2024Article
- Development of a potent embryonic chick lens model for studying congenital cataracts in vivo.Communications biology · 2021Article
- Cas9-expressing chickens and pigs as resources for genome editing in livestock.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.Biology open · 2020Article
- Applications of Gene Editing in Chickens: A New Era Is on the Horizon.Frontiers in genetics · 2018Review
- BarTeL, a Genetically Versatile, Bioluminescent and Granule Neuron Precursor-Targeted Mouse Model for Medulloblastoma.PloS one · 2016Article
- Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads.Developmental biology · 2014Article
- Use of lentiviral vectors to deliver and express bicistronic transgenes in developing chicken embryos.Methods (San Diego, Calif.) · 2014Article
- Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development.Molecular biology of the cell · 2014Article
- Overexpression of aromatase alone is sufficient for ovarian development in genetically male chicken embryos.PloS one · 2013Article
- Production of high-titer RCAS retrovirus.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Article
- Receptor for hyaluronan-mediated motility isoform B promotes liver metastasis in a mouse model of multistep tumorigenesis and a tail vein assay for metastasis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A common progenitor for retinal astrocytes and oligodendrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors.The Journal of comparative neurology · 2010Article
- Pro-photoreceptor activity of chick neurogenin1.Investigative ophthalmology & visual science · 2009Article
- A right-sided pathway involving FGF8/Snai1 controls asymmetric development of the proepicardium in the chick embryo.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Effect of bone morphogenetic protein signaling on development of the jaw skeleton.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Article
- Murine retroviruses re-engineered for lineage tracing and expression of toxic genes in the developing chick embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Article
- Retinal progenitor cells can produce restricted subsets of horizontal cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication-competent avian retroviruses, capable of transducing and expressing up to 2 kb of nonviral sequences, are now available to effect widespread gene transfer in chicken (chick) embryos (S. H. Hughes, J. J. Greenhouse, C. J. Petropoulos, and P. Sutrave, J. Virol. 61:3004-3012, 1987). We have constructed novel avian retroviral vectors that encode human placental alkaline phosphatase as a marker whose expression can be histochemically monitored. These vectors have been tested for expression by introducing them into the embryonic chick nervous system. They have revealed that the expression of retrovirally transduced genes can be spatially and temporally limited without the need for tissue-specific promoters. By varying the site and time of infection, targeted gene transfer can be confined to selected populations of neural cells over the course of several days, a time window that is sufficient for many key developmental processes. The capability of differentially infecting specific target populations may avoid confounding variables such as detrimental effects of a transduced gene on processes unrelated to the cells or tissue of interest. These vectors and methods thus should be useful in studies of the effect of transduced genes on the development of various organs and tissues during avian embryogenesis. In addition, the vectors will facilitate studies aimed at an understanding of viral infection and expression patterns.
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