ArticleMolecular and cellular biology1993
GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 144 citations in OpenAlex.
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- Transcriptomic Analysis of the Interaction Between Sake Yeast and Kuratsuki Bacillus in Co-Culture.Current microbiology · 2025Article
- A nuclear lamina-chromatin-Ran GTPase axis modulates nuclear import and DNA damage signaling.Aging cell · 2019Article
- Identification of Genes inG3 (Bethesda, Md.) · 2017Article
- Characterization of a Ran gene from Puccinia striiformis f. sp. tritici involved in fungal growth and anti-cell death.Scientific reports · 2016Article
- Altering nuclear pore complex function impacts longevity and mitochondrial function in S. cerevisiae.The Journal of cell biology · 2015Article
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- Targeting of the SUN protein Mps3 to the inner nuclear membrane by the histone variant H2A.Z.The Journal of cell biology · 2011Article
- Conjugated linoleic acid inhibits hyphal growth in Candida albicans by modulating Ras1p cellular levels and downregulating TEC1 expression.Eukaryotic cell · 2011Article
- Overexpression of OsRAN2 in rice and Arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress.Journal of experimental botany · 2010Article
- Ran overexpression leads to diminished T cell responses and selectively modulates nuclear levels of c-Jun and c-Fos.The Journal of biological chemistry · 2010Article
- Lte1, Cdc14 and MEN-controlled Cdk inactivation in yeast coordinate rDNA decompaction with late telophase progression.The EMBO journal · 2009Article
- RanGTPase: A Key Regulator of Nucleocytoplasmic Trafficking.Molecular and cellular pharmacology · 2009Article
- Functional analysis of key nuclear trafficking components reveals an atypical Ran network required for parasite pathogenesis.Molecular microbiology · 2008Article
- Regulation of the transcriptional response to oxidative stress in fungi: similarities and differences.Eukaryotic cell · 2003Review
- The Ran GTPase cycle is required for yeast nuclear pore complex assembly.The Journal of cell biology · 2003Article
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- A protein required for nuclear-protein import, Mog1p, directly interacts with GTP-Gsp1p, the Saccharomyces cerevisiae ran homologue.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA.Molecular and cellular biology · 1998Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The temperature-sensitive mutation prp20-1 of Saccharomyces cerevisiae exhibits a pleiotropic phenotype associated with a general failure to maintain a proper organization of the nucleus. Its mammalian homolog, RCC1, is not only reported to be involved in the negative control of chromosome condensation but is also believed to assist in the coupling of DNA replication to the entry into mitosis. Recent studies on Xenopus RCC1 have strongly suggested a further role for this protein in the formation or maintenance of the DNA replication machinery. To elucidate the nature of the various components required for this PRP20 control pathway in S. cerevisiae, we undertook a search for multicopy suppressors of a prp20 thermosensitive mutant. Two genes, GSP1 and GSP2, were identified that encode almost identical polypeptides of 219 and 220 amino acids. Sequence analyses of these proteins show them to contain the ras consensus domains involved in GTP binding and metabolism. The levels of the GSP1 transcript are about 10-fold those of GSP2. As for S. cerevisiae RAS2, GSP2 expression exhibits carbon source dependency, while GSP1 expression does not. GSP1 is an essential gene, and GSP2 is not required for cell viability. We show that GSP1p is nuclear, that it can bind GTP in an in vitro assay, and finally, that a mutation in GSP1p which activates small ras-like proteins by increasing the stability of the GTP-bound form causes a dominant lethal phenotype. We believe that these two gene products may serve in regulating the activities of the multicomponent PRP20 complex.
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