ArticleInfection and immunity1993
Roles of Ca2+ and F-actin in intracellular aggregation of Chlamydia trachomatis in eucaryotic cells.
Article in Infection and immunity, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- Endoplasmic reticulum: the target of chlamydial manipulation.Archives of microbiology · 2025Review
- Gynecological Cancers and Microbiota Dynamics: Insights into Pathogenesis and Therapy.International journal of molecular sciences · 2024Review
- Epidermal Growth Factor Receptor and Transforming Growth Factor β Signaling Pathways Cooperate To MediateInfection and immunity · 2020Article
- Article
- Activation of epidermal growth factor receptor is required for Chlamydia trachomatis development.BMC microbiology · 2014Article
- Rab GTPases are recruited to chlamydial inclusions in both a species-dependent and species-independent manner.Infection and immunity · 2003Article
- Chlamydia trachomatis disrupts N-cadherin-dependent cell-cell junctions and sequesters beta-catenin in human cervical epithelial cells.Infection and immunity · 2002Article
- Rapid, transient phosphatidylserine externalization induced in host cells by infection with Chlamydia spp.Infection and immunity · 2001Article
- Campylobacter jejuni 81-176 associates with microtubules and dynein during invasion of human intestinal cells.Infection and immunity · 1999Article
- Fusion of Chlamydia trachomatis-containing inclusions is inhibited at low temperatures and requires bacterial protein synthesis.Infection and immunity · 1998Article
- Host cell phospholipids are trafficked to and then modified by Chlamydia trachomatis.Journal of bacteriology · 1997Article
- Phagosome-lysosome fusion is a calcium-independent event in macrophages.The Journal of cell biology · 1996Article
- Cytoskeletal requirements in Chlamydia trachomatis infection of host cells.Infection and immunity · 1995Article
- Chlamydia trachomatis serovar L2 induces protein tyrosine phosphorylation during uptake by HeLa cells.Infection and immunity · 1994Article
- Selective translocation of annexins during intracellular redistribution of Chlamydia trachomatis in HeLa and McCoy cells.Infection and immunity · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of intracellular free Ca2+ ([Ca2+]i) on the intracellular aggregation of Chlamydia trachomatis serovars L2 and E in McCoy and HeLa cells is investigated. Loading the cells with the Ca2+ chelator MAPT/AM (1,2-bis-5-methyl-amino-phenoxylethane-N,N-n'-tetra-acetoxymethyl acetate), thereby decreasing the [Ca2+]i from 67 to 19 nM, decreased the number of cells with a local aggregation of chlamydiae in a dose-dependent manner. Neither the attachment nor the uptake of elementary bodies (EBs) was, however, affected after depletion of Ca2+ from the cells. There was no significant difference in the level of measured [Ca2+]i between infected and uninfected cells. Reducing the [Ca2+]i also significantly inhibited chlamydial inclusion formation. Differences in the organization of the actin filament network were observed in response to [Ca2+]i depletion. In Ca(2+)-depleted cells, where few EB aggregates were formed, few local accumulations of F-actin were observed in the cytosol. These results suggest that the aggregation of EBs in eucaryotic cells requires a normal homeostasis of intracellular Ca2+. By affecting F-actin reorganization and putatively certain Ca(2+)-binding proteins, [Ca2+]i plays a vital role in the infectious process of chlamydiae.
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