ArticleNucleic acids research1993
Functional analysis of a C. elegans trans-splice acceptor.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- N-Ethyl-N-Nitrosourea (ENU) Mutagenesis Reveals an Intronic Residue Critical for Caenorhabditis elegans 3' Splice Site Function in Vivo.G3 (Bethesda, Md.) · 2016Article
- Cross-validated methods for promoter/transcription start site mapping in SL trans-spliced genes, established using the Ciona intestinalis troponin I gene.Nucleic acids research · 2011Article
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- U2AF binding selects for the high conservation of the C. elegans 3' splice site.RNA (New York, N.Y.) · 2005Article
- Spliced-leader RNA trans splicing in a chordate, Oikopleura dioica, with a compact genome.Molecular and cellular biology · 2004Article
- Intercistronic region required for polycistronic pre-mRNA processing in Caenorhabditis elegans.Molecular and cellular biology · 2001Article
- SURVEY AND SUMMARY: exon-intron organization of genes in the slime mold Physarum polycephalum.Nucleic acids research · 2000Article
- U2AF35 is encoded by an essential gene clustered in an operon with RRM/cyclophilin in Caenorhabditis elegans.RNA (New York, N.Y.) · 1999Article
- SL1 trans splicing and 3'-end formation in a novel class of Caenorhabditis elegans operon.Molecular and cellular biology · 1999Article
- Intronic and exonic sequences modulate 5' splice site selection in plant nuclei.Nucleic acids research · 1997Article
- Cloning of Caenorhabditis U2AF65: an alternatively spliced RNA containing a novel exon.Molecular and cellular biology · 1997Article
- Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery.Plant molecular biology · 1996Review
- Characterization of intronic uridine-rich sequence elements acting as possible targets for nuclear proteins during pre-mRNA splicing in Nicotiana plumbaginifolia.Nucleic acids research · 1996Article
- Phylogeny and structure of the RING3 gene.Immunogenetics · 1996Article
- Experimental evidence for RNA trans-splicing in mammalian cells.The EMBO journal · 1995Article
- AU-rich intronic elements affect pre-mRNA 5' splice site selection in Drosophila melanogaster.Molecular and cellular biology · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The rol-6 gene is trans-spliced to the 22 nt leader, SL1, 173 nt downstream of the transcription start. We have analyzed splicing in transformants carrying extrachromosomal arrays of rol-6 with mutations in the trans-splice acceptor site. This site is a close match to the consensus, UUUCAG, that is highly conserved in both trans-splice and intron acceptor sites in C. elegans. When the trans-splice site was inactivated by mutating the perfectly-conserved AG, trans-splicing still occurred, but at a cryptic site 20 nt upstream. We tested the frequency with which splicing switched from the normal site to the cryptic site when the pyrimidines at this site were changed to A's. Since most C. elegans 3' splice sites lack an obvious polypyrimidine tract, we hypothesized that these four pyrimidines might play this role, and indeed mutation of these bases caused splicing to switch to the cryptic site. We also demonstrated that a major reason the downstream site is normally favored is because it occurs at a boundary between A+U rich and non-A+U rich RNA. When the RNA between the two splice sites was made less A+U rich, splicing occurred preferentially at the upstream site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.