ArticleAmerican journal of human genetics1993
Libraries for each human chromosome, constructed from sorter-enriched chromosomes by using linker-adaptor PCR.
Article in American journal of human genetics, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Bioinformatics tools allow targeted selection of chromosome enumeration probes and aneuploidy detection.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Article
- Genetic analysis using genomic representations.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Region-specific YAC banding and painting probes for comparative genome mapping: implications for the evolution of human chromosome 2.Chromosoma · 1996Article
- Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based chromosomal homologies.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Zoo-FISH delineates conserved chromosomal segments in horse and man.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
- Generation of chromosome fragment specific bovine DNA sequences by microdissection and DOP-PCR.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- A comparative map of the porcine and human genomes demonstrates ZOO-FISH and gene mapping-based chromosomal homologies.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Homologous and heterologous FISH painting with PARM-PCR chromosome-specific probes in mammals.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Partial disomy of Xp and the presence of SRY in a phenotypic female.Journal of medical genetics · 1995Article
- Transforming growth factors-α and -β expression in fertilized and parthenogenetic pre-implantation mouse embryos: RNA detection with fluorescent in situ hybridization.Development, growth & differentiation · 1995Article
- Pure chromosome-specific PCR libraries from single sorted chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We describe here the production of complex libraries enriched in sequences from each human chromosome type, starting with only a few thousand sorter-purified chromosomes. In this procedure, DNA is extracted from the sorted chromosomes, digested to completion by using the frequently cutting restriction endonuclease Sau3A1, and ligated, on each end, to an adaptor oligonucleotide. These fragments are then amplified using PCR with a sequence homologous to the adaptor oligonucleotide as a primer. We have used this procedure to produce PCR libraries for each of the 24 human chromosomes. These libraries were characterized by gel electrophoresis and found to be composed of a continuum of sequences ranging in size from a few hundred to approximately 1,000 bp. The libraries, when used as probes for fluorescence in situ hybridization, stained the target chromosomes more or less continuously, even after PCR amplification for more than 200 cycles. These libraries are useful as hybridization probes to facilitate molecular cytogenetic studies and as sources of probes either for identification of polymorphic short tandemly repeated sequences or for development of sequence-tagged sites.
Indexed as
Identifiers
8447324PMC1682175What OpenQuestion holds
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