ArticleJournal of virology1993
Characterization of mutants within the gene for the adenovirus E3 14.7-kilodalton protein which prevents cytolysis by tumor necrosis factor.
Article in Journal of virology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Biographical Feature: William S. M. Wold, Ph.D., 1944-2021.Journal of virology · 2021Article
- E3-14.7K is recruited to TNF-receptor 1 and blocks TNF cytolysis independent from interaction with optineurin.PloS one · 2012Article
- Expression of active p21-activated kinase-1 induces Ca2+ flux modification with altered regulatory protein phosphorylation in cardiac myocytes.American journal of physiology. Cell physiology · 2009Article
- Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit.Journal of virology · 2007Article
- Inhibition of TNF receptor 1 internalization by adenovirus 14.7K as a novel immune escape mechanism.The Journal of clinical investigation · 2006Article
- Cytokine response and survival of mice immunized with an adenovirus expressing Bacillus anthracis protective antigen domain 4.Infection and immunity · 2006Article
- Functional interactions of antiapoptotic proteins and tumor necrosis factor in the context of a replication-competent adenovirus.Gene therapy · 2005Article
- Transforming growth factor beta1 receptor II is downregulated by E1A in adenovirus-infected cells.Journal of virology · 2003Article
- p53 alone or in combination with antisense cyclin D1 induces apoptosis and reduces tumor size in human melanoma.Cancer gene therapy · 2002Article
- Inhibition of tumor necrosis factor alpha-induced NF-kappa B activation by the adenovirus E3-10.4/14.5K complex.Journal of virology · 2002Article
- Characterization of Ad5 E3-14.7K, an adenoviral inhibitor of apoptosis: structure, oligomeric state, and metal binding.Protein science : a publication of the Protein Society · 2002Article
- Inhibition of TRAIL-induced apoptosis and forced internalization of TRAIL receptor 1 by adenovirus proteins.Journal of virology · 2001Article
- E-cadherin expression in melanoma cells restores keratinocyte-mediated growth control and down-regulates expression of invasion-related adhesion receptors.The American journal of pathology · 2000Article
- Review
- Adenovirus E3-10.4K/14.5K protein complex inhibits tumor necrosis factor-induced translocation of cytosolic phospholipase A2 to membranes.Journal of virology · 1997Article
- The adenovirus E3-14.7K protein and the E3-10.4K/14.5K complex of proteins, which independently inhibit tumor necrosis factor (TNF)-induced apoptosis, also independently inhibit TNF-induced release of arachidonic acid.Journal of virology · 1996Article
- The adenovirus death protein (E3-11.6K) is required at very late stages of infection for efficient cell lysis and release of adenovirus from infected cells.Journal of virology · 1996Article
- Region E3 of subgroup B human adenoviruses encodes a 16-kilodalton membrane protein that may be a distant analog of the E3-6.7K protein of subgroup C adenoviruses.Journal of virology · 1995Article
- Characterization of transgenic mice containing adenovirus early region 3 genomic DNA.Journal of virology · 1994Article
- The adenovirus E3 14.7-kilodalton protein which inhibits cytolysis by tumor necrosis factor increases the virulence of vaccinia virus in a murine pneumonia model.Journal of virology · 1994Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The 14,700-Da protein (14.7K protein) encoded by the E3 region of adenovirus has previously been shown to protect mouse cells from cytolysis by tumor necrosis factor (TNF). Delineating the sequences in the 14.7K protein that are required for this activity may provide insight into the mechanism of protection from TNF by 14.7K as well as the mechanism of TNF cytolysis. In the present study, we examined the ability of 14.7K mutants to protect cells from lysis by TNF. In-frame deletions as well as Cys-to-Ser mutations in the 14.7K gene were generated by site-directed mutagenesis and then built into the genome of a modified adenovirus type 5 (dl7001) that lacks all E3 genes. dl7001, which replicates to the same titers as does adenovirus type 5 in cultured cells, has the largest E3 deletion analyzed to date. 51Cr release was used to assay TNF cytolysis. Our results indicate that most mutations in the 14.7K gene result in a loss of function, suggesting that nearly the entire protein rather than a specific domain functions to prevent TNF cytolysis.
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