ArticleDigestive diseases and sciences1993
Contraction of collagen gels by intestinal epithelial cells depends on microfilament function.
Article in Digestive diseases and sciences, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Gut homeostasis, injury, and healing: New therapeutic targets.World journal of gastroenterology · 2022Review
- Cellular polarity modulates drug resistance in primary colorectal cancers via orientation of the multidrug resistance protein ABCB1.The Journal of pathology · 2019Article
- Article
- Selective induction of integrin beta1 by hypoxia-inducible factor: implications for wound healing.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- MUC1 expressed in PanC1 cells decreases adhesion to type 1 collagen but increases contraction in collagen lattices.The American journal of pathology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a model system to quantify the tractional forces generated by intestinal epithelial cells during organization into a confluent epithelial cell sheet. In this model system, IEC-6 cells, a rat intestinal crypt cell line, rapidly contracted collagen gels reducing the gel surface area by 97% at 24 hr. The tractional forces measured by gel contraction were directly related to the number of cells added and were inversely related to the collagen concentration of the gel. Actin microfilament function was required for gel contraction, but microtubular function was not. Fetal bovine serum and protein synthesis were required for maximal gel contraction. IEC-6 (5 x 10(5)) cells per gel and fibroblasts (5 x 10(4)) cells added to collagen gels resulted in contraction of the gels by 50% at 24 hr. Therefore, intestinal epithelial cells and fibroblasts generate tractional forces of similar strength capable of organizing the surrounding extracellular matrix, which should be considered in models of intestinal morphogenesis and repair.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.