ArticleMolecular biology of the cell1993
Cell cycle expression of two replicative DNA polymerases alpha and delta from Schizosaccharomyces pombe.
Article in Molecular biology of the cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Rad4TopBP1, a scaffold protein, plays separate roles in DNA damage and replication checkpoints and DNA replication.Molecular biology of the cell · 2006Article
- Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.Eukaryotic cell · 2005Article
- The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.Molecular and cellular biology · 2004Article
- Replication proteins influence the maintenance of telomere length and telomerase protein stability.Molecular and cellular biology · 2003Article
- Role of fission yeast primase catalytic subunit in the replication checkpoint.Molecular biology of the cell · 2001Article
- Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation.Molecular and cellular biology · 2000Article
- Factors controlling cyclin B expression.Plant molecular biology · 2000Review
- Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21(Cip1)-like PCNA binding motif.The EMBO journal · 2000Article
- DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27.The EMBO journal · 1996Article
- Studies of Schizosaccharomyces pombe DNA polymerase alpha at different stages of the cell cycle.Nucleic acids research · 1995Article
- Suppressors of thermosensitive mutations in the DNA polymerase delta gene of Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1995Article
- Transcriptional regulation of DNA replication-related genes in cell growth, differentiation and oncogenesis.Japanese journal of cancer research : Gann · 1994Review
- In vivo species specificity of DNA polymerase alpha.Molecular & general genetics : MGG · 1993Article
- Fission yeast with DNA polymerase delta temperature-sensitive alleles exhibits cell division cycle phenotype.Nucleic acids research · 1993Article
- MluI site-dependent transcriptional regulation of the Candida albicans dUTPase gene.Current geneticsArticle
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3 authors at 1 institution in 1 country.
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Abstract
We have investigated the expression of two Schizosaccharomyces pombe replicative DNA polymerases alpha and delta during the cell cycle. The pol alpha+ and pol delta+ genes encoding DNA polymerases alpha and delta were isolated from S. pombe. Both pol alpha+ and pol delta+ genes are single copy genes in haploid cells and are essential for cell viability. In contrast to Saccharomyces cerevisiae homologs, the steady-state transcripts of both S. pombe pol alpha+ and pol delta+ genes were present throughout the cell cycle. Sequence analysis of the pol alpha+ and pol delta+ genes did not reveal the Mlu I motifs in their upstream sequences that are involved in cell cycle-dependent transcription of S. cerevisiae DNA synthesis genes as well as the S. pombe cdc22+ gene at the G1/S boundary. However, five near-match Mlu I motifs were found in the upstream region of the pol alpha+ gene. S. pombe DNA polymerases alpha and delta proteins were also expressed constantly throughout the cell cycle. In addition, the enzymatic activity of the S. pombe DNA polymerase alpha measured by in vitro assay was detected at all stages of the cell cycle. Thus, these S. pombe replicative DNA polymerases, like that of S. pombe cdc17+ gene, are expressed throughout the cell cycle at the transcriptional and protein level. These results indicate that S. pombe has at least two regulatory modes for the expression of genes involved in DNA replication and DNA precursor synthesis.
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