ArticleMolecular and cellular biology1993
T cell receptor-beta mRNA splicing: regulation of unusual splicing intermediates.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Cross talk between immunoglobulin heavy-chain transcription and RNA surveillance during B cell development.Molecular and cellular biology · 2012Article
- Frame-disrupting mutations elicit pre-mRNA accumulation independently of frame disruption.Nucleic acids research · 2010Article
- Nuclear translation: what is the evidence?RNA (New York, N.Y.) · 2003Review
- T-cell receptor sequences that elicit strong down-regulation of premature termination codon-bearing transcripts.The EMBO journal · 2002Article
- Nonsense-mediated decay of glutathione peroxidase 1 mRNA in the cytoplasm depends on intron position.The EMBO journal · 2000Article
- The stability and fate of a spliced intron from vertebrate cells.RNA (New York, N.Y.) · 1999Article
- At least one intron is required for the nonsense-mediated decay of triosephosphate isomerase mRNA: a possible link between nuclear splicing and cytoplasmic translation.Molecular and cellular biology · 1998Article
- A mutated human homologue to yeast Upf1 protein has a dominant-negative effect on the decay of nonsense-containing mRNAs in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Binary specification of nonsense codons by splicing and cytoplasmic translation.The EMBO journal · 1998Article
- Distal V beta promoters transcribe novel T-cell receptor-beta transcripts in early development.Immunology · 1998Article
- T cell receptor (TCR) mini-gene mRNA expression regulated by nonsense codons: a nuclear-associated translation-like mechanism.The Journal of experimental medicine · 1997Article
- Evidence that translation reinitiation abrogates nonsense-mediated mRNA decay in mammalian cells.The EMBO journal · 1997Article
- A splicing-dependent regulatory mechanism that detects translation signals.The EMBO journal · 1996Article
- Effects of nonsense mutations on nuclear and cytoplasmic adenine phosphoribosyltransferase RNA.Molecular and cellular biology · 1996Article
- Defects in RNA splicing and the consequence of shortened translational reading frames.American journal of human genetics · 1996Article
- Deletion of the mouse T-cell receptor beta gene enhancer blocks alphabeta T-cell development.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Nonstop treatment of cystic fibrosis.Nature medicine · 1996Article
- Evidence that the decay of nucleus-associated nonsense mRNA for human triosephosphate isomerase involves nonsense codon recognition after splicing.RNA (New York, N.Y.) · 1996Article
- Translation initiation of ornithine decarboxylase and nucleocytoplasmic transport of cyclin D1 mRNA are increased in cells overexpressing eukaryotic initiation factor 4E.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- When cells stop making sense: effects of nonsense codons on RNA metabolism in vertebrate cells.RNA (New York, N.Y.) · 1995Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The expression of functional T cell receptor-beta (TCR-beta) transcripts requires the activation of programmed DNA rearrangement events. It is not clear whether other mechanisms dictate TCR-beta mRNA levels during thymic ontogeny. We examined the potential role of RNA splicing as a regulatory mechanism. As a model system, we used an immature T cell clone, SL12.4, that transcribes a fully rearranged TCR-beta gene but essentially lacks mature 1.3-kb TCR-beta transcripts in the cytoplasm. Abundant TCR-beta splicing intermediates accumulate in the nucleus of this cell clone. These splicing intermediates result from inefficient or inhibited excision of four of the five TCR-beta introns; the only intron that is efficiently spliced is the most 5' intron, IVSL. The focal point for the regulation appears to be IVS1C beta 1 and IVS2C beta 1, since unusual splicing intermediates that have cleaved the 5' splice site but not the 3' splice site of these two introns accumulate in vivo. The block in 3' splice site cleavage is of interest since sequence analysis reveals that these two introns possess canonical splice sites. A repressional mechanism involving a labile repressor protein may be responsible for the inhibition of RNA splicing since treatment of SL12.4 cells with the protein synthesis inhibitor cycloheximide reversibly induces a rapid and dramatic accumulation of fully spliced TCR-beta transcripts in the cytoplasm, concomitant with a decline in TCR-beta pre-mRNAs in the nucleus. This inducible system may be useful for future studies analyzing the underlying molecular mechanisms that regulate RNA splicing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.