ArticleMolecular and cellular biology1993
The human prointerleukin 1 beta gene requires DNA sequences both proximal and distal to the transcription start site for tissue-specific induction.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 178 citations in OpenAlex.
- Article
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- The Interleukin-1 (IL-1) Superfamily Cytokines and Their Single Nucleotide Polymorphisms (SNPs).Journal of immunology research · 2022Review
- First insights into the molecular basis association between promoter polymorphisms of the IL1B gene and Helicobacter pylori infection in the Sudanese population: computational approach.BMC microbiology · 2021Article
- Immunomodulatory Effects of Azithromycin Revisited: Potential Applications to COVID-19.Frontiers in immunology · 2021Review
- Transcriptional Regulation of Inflammasomes.International journal of molecular sciences · 2020Review
- Review
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- Development of a novel cell-based assay to diagnose recurrent focal segmental glomerulosclerosis patients.Kidney international · 2019Article
- A combined computational and experimental approach reveals the structure of a C/EBPβ-Spi1 interaction required forThe Journal of biological chemistry · 2018Article
- Distinct mechanisms regulate IL1B gene transcription in lymphoid CD4 T cells and monocytes.Cytokine · 2018Article
- Development of In Vivo Imaging Tools for Investigating Astrocyte Activation in Epileptogenesis.Molecular neurobiology · 2018Article
- MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ.PloS one · 2017Article
- CXCL8, IL-1β and sCD200 are pro-inflammatory cytokines and their levels increase in the circulation of breast carcinoma patients.Biomedical reports · 2016Article
- Association of interleukin 1 beta (IL-1β) polymorphism with mRNA expression and risk of non small cell lung cancer.Meta gene · 2014Article
- Interactions between pork consumption, CagA status and IL-1B-31 genotypes in gastric cancer.World journal of gastroenterology · 2014Article
- Identification and expression profiles of prdm1 in medaka Oryzias latipes.Molecular biology reports · 2014Article
- Distinct mechanisms for induction and tolerance regulate the immediate early genes encoding interleukin 1β and tumor necrosis factor α.PloS one · 2013Article
- Association of the interleukin 1 beta gene and brain spontaneous activity in amnestic mild cognitive impairment.Journal of neuroinflammation · 2012Article
- Interleukin-1β in Central Nervous System Injury and Repair.European journal of neurodegenerative disease · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In these studies, we have identified DNA sequences and specific protein interactions necessary for transcriptional regulation of the human prointerleukin 1 beta (proIL-1 beta) gene. A cell-type-independent lipopolysaccharide (LPS)-responsive enhancer element located between -3757 and -2729 bp upstream from the transcription start site (cap site) consisted of at least six discrete subregions which were essential to the maximal induction by LPS in transfected monocytes. The enhancer also appeared to mediate phorbol myristate acetate induction in monocytes and IL-1 responsiveness in fibroblasts. Deletion and base substitution mutations along with DNA binding studies demonstrated that the enhancer contained a minimum of three functional protein binding sequences, two of which appeared to be important for gene induction. One of the essential proteins which bound to the enhancer was similar or identical to members of the C/EBP family of transcription factors required for both IL-1- and LPS-specific induction of the IL-6 gene (i.e., the NF-IL6 proteins). When ligated to the proIL-1 beta cap site-proximal region (located between -131 to +12), both the proIL-1 beta and the simian virus 40 enhancer elements functioned more efficiently in monocytes than in HeLa cells, which are not normally competent for IL-1 beta expression. When ligated to the murine c-fos promoter, however, the proIL-1 beta enhancer was inducible in phorbol myristate acetate-stimulated HeLa cells, suggesting the existence of a proIL-1 beta promoter-proximal requirement for tissue specificity.
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