ArticleMolecular and cellular biology1993
Factors affecting authentic 5' splice site selection in plant nuclei.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- A genetic screen in Arabidopsis reveals the identical roles for RBP45d and PRP39a in 5' cryptic splice site selection.Frontiers in plant science · 2022Article
- A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing.International journal of molecular sciences · 2020Article
- A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time.Journal of experimental botany · 2009Article
- Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.).The Plant journal : for cell and molecular biology · 2008Article
- Identification of the photorespiratory 2-phosphoglycolate phosphatase, PGLP1, in Arabidopsis.Plant physiology · 2007Article
- Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Computational comparative analyses of alternative splicing regulation using full-length cDNA of various eukaryotes.RNA (New York, N.Y.) · 2004Article
- Article
- Splicing of the maize Sh1 first intron is essential for enhancement of gene expression, and a T-rich motif increases expression without affecting splicing.Plant physiology · 2002Article
- Mutational analysis of a plant branchpoint and polypyrimidine tract required for constitutive splicing of a mini-exon.RNA (New York, N.Y.) · 2002Article
- Molecular structure of three mutations at the maize sugary1 locus and their allele-specific phenotypic effects.Plant physiology · 2001Article
- RBP45 and RBP47, two oligouridylate-specific hnRNP-like proteins interacting with poly(A)+ RNA in nuclei of plant cells.RNA (New York, N.Y.) · 2000Article
- UBP1, a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation.The EMBO journal · 2000Article
- A splice site mutant of maize activates cryptic splice sites, elicits intron inclusion and exon exclusion, and permits branch point elucidation.Plant physiology · 1999Article
- The AG dinucleotide terminating introns is important but not always required for pre-mRNA splicing in the maize endospermPlant physiology · 1999Article
- An intragenic suppressor of the Arabidopsis floral organ identity mutant apetala3-1 functions by suppressing defects in splicing.The Plant cell · 1998Article
- G triplets located throughout a class of small vertebrate introns enforce intron borders and regulate splice site selection.Molecular and cellular biology · 1997Article
- Intronic and exonic sequences modulate 5' splice site selection in plant nuclei.Nucleic acids research · 1997Article
- Logitlinear models for the prediction of splice sites in plant pre-mRNA sequences.Nucleic acids research · 1996Article
- Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery.Plant molecular biology · 1996Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To define elements critical for 5' splice selection in dicot plant nuclei, wild-type and mutant transcripts containing the first intron of the pea rbcS3A gene were expressed in vivo by using an autonomously replicating plant expression vector. Mutations within the normal 5' splice site (+1) of this intron demonstrate that 5' splice sites at the normal exon-intron boundary having only limited agreement with a 5' splice site consensus sequence can be spliced quite effectively in dicot nuclei. Inactivation of the normal 5' splice site occurs only by point mutations of the G at position +1 of the intron (+1G) or +2U or by multiple mutations at other positions and results in the activation of three cryptic 5' splice sites in the adjacent exon and intron. cis competition of cryptic sites having consensus 5' splice site sequences with the normal 5' splice site demonstrates that cryptic splice sites in the exon, but not the intron, can compete to some extent with the normal site. Replacement of the sequences between the cryptic and normal 5' splice sites with heterologous exon or intron sequences demonstrates that the 5' boundary of this plant intron is defined by its position relative to the AU transition point between exon and intron. These results suggest that potential 5' splice sites upstream of the AU transition point are accessible for recognition by the plant pre-mRNA splicing machinery and that those downstream in the AU-rich intron are masked from recognition.
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