ArticleThe Journal of membrane biology1993
Anomalously slow mobility of fluorescent lipid probes in the plasma membrane of the yeast Saccharomyces cerevisiae.
Article in The Journal of membrane biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae.Journal of molecular biology · 2020Article
- Periprotein lipidomes ofeLife · 2020Article
- Endoplasmic reticulum-plasma membrane contact sites integrate sterol and phospholipid regulation.PLoS biology · 2018Article
- Budding Yeast: An Ideal Backdrop forFrontiers in cell and developmental biology · 2016Review
- Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Compartmentalized nodes control mitotic entry signaling in fission yeast.Molecular biology of the cell · 2013Article
- Protein diffusion in plant cell plasma membranes: the cell-wall corral.Frontiers in plant science · 2013Article
- Non-uniform membrane diffusion enables steady-state cell polarization via vesicular trafficking.Nature communications · 2013Article
- Patchwork organization of the yeast plasma membrane into numerous coexisting domains.Nature cell biology · 2012Article
- Osh proteins regulate membrane sterol organization but are not required for sterol movement between the ER and PM.Traffic (Copenhagen, Denmark) · 2011Article
- A method improving the accuracy of fluorescence recovery after photobleaching analysis.Biophysical journal · 2008Article
- Ethanol/naltrexone interactions at the mu-opioid receptor. CLSM/FCS study in live cells.PloS one · 2008Article
- Unrestricted diffusion of exogenous and endogenous PIP(2 )in baby hamster kidney and Chinese hamster ovary cell plasmalemma.The Journal of membrane biology · 2007Article
- Endocytosis optimizes the dynamic localization of membrane proteins that regulate cortical polarity.Cell · 2007Article
- Reticulated lipid probe fluorescence reveals MDCK cell apical membrane topography.Biophysical journal · 2002Article
- Lateral diffusion in planar lipid bilayers: a fluorescence recovery after photobleaching investigation of its modulation by lipid composition, cholesterol, or alamethicin content and divalent cations.Biophysical journal · 1996Article
- The plasma membrane of Saccharomyces cerevisiae: structure, function, and biogenesis.Microbiological reviews · 1995Review
- Analysis of simulated and experimental fluorescence recovery after photobleaching. Data for two diffusing components.Biophysical journal · 1995Article
- Cheek cell membrane fluidity measured by fluorescence recovery after photobleaching and steady-state fluorescence anisotropy.The Journal of membrane biology · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We measured the lateral mobility of two fluorescent lipid probes dioctadecylindocarbocyanine (diI) and tetramethyl rhodamine phosphatidylethanolamine (R-PE) in the plasma membranes of Saccharomyces cerevisiae ino1 and opi3 spheroplasts. These are well-characterized strains with mutations in the inositol and phosphatidylcholine biosynthetic pathways. Membrane phospholipid composition was altered by growing these mutants in the presence or absence of inositol and choline. Lateral mobility was measured by fluorescence recovery after photobleaching (FRAP). Microscopic fluorescence polarization employing CCD digital imaging produced an ordered orientation distribution of the lipid probe diI, confirming that at least one of the probes was largely incorporated into the bilayer membrane. Our results demonstrated anomalously slow mobility of both lipid probes for both mutants, regardless of whether the lipid composition was near normal or dramatically altered in relative composition of phosphatidylinositol and phosphatidylcholine. Trypsinization of the spheroplasts to remove surface proteins resulted in markedly increased lateral mobility. However, even in trypsinized spheroplasts, mobility was still somewhat lower than the mobility observed in the membrane of mammalian cells, such as rat smooth muscle culture cells tested here for comparison.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.