ArticleThe EMBO journal1993
The Drosophila doublesex proteins share a novel zinc finger related DNA binding domain.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed, 241 citations in OpenAlex.
- Comparative Genomics of the Endosymbiont Cardinium Causing Reproductive Manipulation in Encarsia Parasitoid Wasps.MicrobiologyOpen · 2025Article
- Using a female-specific isoform ofiScience · 2025Article
- DMRT1 haploinsufficiency leads to secondary infertility in XY male rabbits†.Biology of reproduction · 2025Article
- The Interaction of DMRTA2 with HSP90β Inhibits p53 Ubiquitination and Activates the p53 Pathway to Suppress the Malignant Progression of Non-Small-Cell Lung Cancer.Current issues in molecular biology · 2025Article
- Understanding the genetics of sex determination in insects and its relevance to genetic pest management.Insect molecular biology · 2025Review
- Challenges in developing a split drive targeting dsx for the genetic control of the invasive malaria vector Anopheles stephensi.Parasites & vectors · 2025Article
- Candidate target genes of the male-specific expressed Doublesex in the termite Reticulitermes speratus.PloS one · 2024Article
- In Tandem Intragenic Duplication of Doublesex and Mab-3-Related Transcription Factor 1 (Genes · 2023Article
- Article
- The function and evolution of a genetic switch controlling sexually dimorphic eye differentiation in honeybees.Nature communications · 2023Article
- A chromosome-level assembly supports genome-wide investigation of the DMRT gene family in the golden mussel (Limnoperna fortunei).GigaScience · 2022Article
- Genomics of sexual cell fate transdifferentiation in the mouse gonad.G3 (Bethesda, Md.) · 2022Article
- Regulation of Doublesex1 Expression for Environmental Sex Determination in the Cladoceran Crustacean Daphnia.Frontiers in cell and developmental biology · 2022Review
- DMRT Transcription Factors in the Control of Nervous System Sexual Differentiation.Frontiers in neuroanatomy · 2022Review
- Multiple Functions of theFrontiers in neuroscience · 2021Review
- Loss of Dmrt5 Affects the Formation of the Subplate and Early Corticogenesis.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Comparative Genomics Studies on theFrontiers in genetics · 2020Article
- Genome-Wide identification of doublesex and Mab-3-Related transcription factor (DMRT) genes in nile tilapia (Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- A subset of brain neurons controls regurgitation in adultThe Journal of experimental biology · 2019Article
- The Hox geneDevelopment (Cambridge, England) · 2019Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The doublesex gene of Drosophila melanogaster is the final member of a well characterized hierarchy of genes that controls somatic sex determination and differentiation. The male-specific and female-specific doublesex polypeptides occupy a terminal position in the hierarchy, and thus regulate those genes responsible for the development of sexually dimorphic characteristics of the fly. To investigate the molecular mechanism by which these two related proteins interact with specific target genes, we have identified and characterized their DNA binding domains. Using gel mobility shift experiments with sequentially deleted polypeptides, site-directed mutagenesis and spectrophotometric assays, we have shown that the two doublesex proteins share a common and novel zinc finger-related DNA binding domain distinct from any reported class of zinc binding proteins. We have further shown that of 10 null dsx alleles, six encode proteins deficient in DNA binding activity, and that three of these alleles are the result of mutations that alter cysteine and histidine residues in the metal binding domain. Our results provide evidence that both the male-specific and female-specific doublesex proteins share and depend upon the same DNA binding domain for function in vivo, suggesting that both proteins bind to, but differentially regulate, a common set of genes in both sexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.