ArticleThe Plant cell1993
Translation of the mRNA of the maize transcriptional activator Opaque-2 is inhibited by upstream open reading frames present in the leader sequence.
Article in The Plant cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 105 citations in OpenAlex.
- From Structure to Function of Promoters and 5'UTRs in Maize.International journal of molecular sciences · 2026Review
- Ribo-Seq Analysis-Based Elucidation of the Dynamic Translation Landscape of Yak Ovarian Tissues in Different Reproductive Stages.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- The Italian Research on the Molecular Characterization of Maize Kernel Development.International journal of molecular sciences · 2022Review
- Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes.BMC plant biology · 2015Article
- Post-transcriptional regulation of gene expression in plants during abiotic stress.International journal of molecular sciences · 2009Review
- Conserved upstream open reading frames in higher plants.BMC genomics · 2008Article
- Wild-type opaque2 and defective opaque2 polypeptides form complexes in maize endosperm cells and bind the opaque2-zein target site.Plant physiology · 2007Article
- Identification of Glu-B1-1 as a candidate gene for the quantity of high-molecular-weight glutenin in bread wheat (Triticum aestivum L.) by means of an association study.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- The maize An2 gene is induced by Fusarium attack and encodes an ent-copalyl diphosphate synthase.Plant molecular biology · 2005Article
- Molecular evolution of the Opaque-2 gene in Zea mays L.Journal of molecular evolution · 2005Article
- Frequency and character of alternative somatic recombination fates of paralogous genes during T-DNA integration.Molecular genetics and genomics : MGG · 2005Article
- Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h.The Plant cell · 2004Article
- A conserved upstream open reading frame mediates sucrose-induced repression of translation.The Plant cell · 2004Article
- Suppression of a ripening-related endo-1,4-beta-glucanase in transgenic pepper fruit does not prevent depolymerization of cell wall polysaccharides during ripening.Plant molecular biology · 2002Article
- A maize r1 gene is regulated post-transcriptionally by differential splicing of its leader.Plant molecular biology · 2002Article
- Inhibitory effect of myb7 uORF on downstream gene expression in homologous (rice) and heterologous (tobacco) systems.Plant molecular biology · 2002Article
- Methylation of the Opaque2 box in zein genes is parent-dependent and affects O2 DNA binding activity in vitro.Plant molecular biology · 2001Article
- Expression of a class 1 knotted1-like homeobox gene is down-regulated in pea compound leaf primordia.Plant molecular biology · 2001Article
- MDH1: an apple homeobox gene belonging to the BEL1 family.Plant molecular biology · 2000Article
- Molecular cloning and expression analysis of the mevalonate kinase gene from Arabidopsis thaliana.Plant molecular biology · 2000Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protein encoded by the Opaque-2 (O2) gene is a transcription factor, translated from an mRNA that possesses an unusually long 5' leader sequence containing three upstream open reading frames (uORFs). The efficiency of translation of O2 mRNA has been tested in vivo by a transient assay in which the level of activation of the b32 promoter, a natural target of O2 protein, is measured. We show that uORF-less O2 alleles possess a higher transactivation value than the wild-type allele and that the reduction in transactivation due to the uORFs is a cis-dominant effect. The data presented indicate that both uORF1 and uORF2 are involved in the reducing effect and suggest that both are likely to be translated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.