ArticleThe Biochemical journal1993
Purification and characterization of a DNA-binding heterodimer of 52 and 100 kDa from HeLa cells.
Article in The Biochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- A novel protein encoded by circCOPA inhibits the malignant phenotype of glioblastoma cells and increases their sensitivity to temozolomide by disrupting the NONO-SFPQ complex.Cell death & disease · 2024Article
- Established and Evolving Roles of the Multifunctional Non-POU Domain-Containing Octamer-Binding Protein (NonO) and Splicing Factor Proline- and Glutamine-Rich (SFPQ).Journal of developmental biology · 2024Review
- Molecular Modelling of NONO and SFPQ Dimerization Process and RNA Recognition Mechanism.International journal of molecular sciences · 2022Article
- Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1.Nucleic acids research · 2022Article
- Kinetic and subcellular analysis of PS-ASO/protein interactions with P54nrb and RNase H1.Nucleic acids research · 2019Article
- Interaction of HIV-1 integrase with polypyrimidine tract binding protein and associated splicing factor (PSF) and its impact on HIV-1 replication.Retrovirology · 2019Article
- Altered stoichiometry and nuclear delocalization of NonO and PSF promote cellular senescence.Aging · 2016Article
- The DBHS proteins SFPQ, NONO and PSPC1: a multipurpose molecular scaffold.Nucleic acids research · 2016Article
- Caenorhabditis elegans NONO-1: Insights into DBHS protein structure, architecture, and function.Protein science : a publication of the Protein Society · 2015Article
- Non-POU Domain-Containing Octamer-Binding Protein Negatively Regulates HIV-1 Infection in CD4(+) T Cells.AIDS research and human retroviruses · 2015Article
- The structure of human SFPQ reveals a coiled-coil mediated polymer essential for functional aggregation in gene regulation.Nucleic acids research · 2015Article
- Arginine methylation and citrullination of splicing factor proline- and glutamine-rich (SFPQ/PSF) regulates its association with mRNA.RNA (New York, N.Y.) · 2015Article
- Paraspeckle nuclear bodies--useful uselessness?Cellular and molecular life sciences : CMLS · 2012Review
- Sequences in PSF/SFPQ mediate radioresistance and recruitment of PSF/SFPQ-containing complexes to DNA damage sites in human cells.DNA repair · 2011Article
- Direct visualization of the co-transcriptional assembly of a nuclear body by noncoding RNAs.Nature cell biology · 2011Article
- Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA.Molecular cell · 2009Article
- Phosphorylation by SR kinases regulates the binding of PTB-associated splicing factor (PSF) to the pre-mRNA polypyrimidine tract.FEBS letters · 2007Article
- p54nrb is a component of the snRNP-free U1A (SF-A) complex that promotes pre-mRNA cleavage during polyadenylation.RNA (New York, N.Y.) · 2006Article
- P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner.Molecular biology of the cell · 2005Article
- Role for PSF in mediating transcriptional activator-dependent stimulation of pre-mRNA processing in vivo.Molecular and cellular biology · 2005Article
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5 authors.
Funding
Abstract
In studies of protein binding to the upstream region of the human proliferation-associated antigen p120 gene, a heterodimer of 52 and 100 kDa proteins was purified from HeLa cells. A 1:1 ratio of p52 and p100 was constant throughout the purification. The heterodimer was localized to cell nuclei, as shown by immunofluorescence. The pI values of the p52 and p100 were 7.8 and 8.6 respectively. The peptide sequences obtained for p52 (QSNKTFNLEKQNHTPRKKHQ and PLRGKQLRVRFAAHSASLTVR) and for p100 (PGGPKPGGGPGLSTPGGHPKPPHRGGGEPPRGRQ and GPGPGQSGPKPPIPPPPPHQQ) were not found in the computer databanks. One p52 peptide sequence, PLRGKQLRVRFA, shows considerable sequence similarity to a conserved motif in topoisomerase II of multiple species. The p52/100 heterodimer bound to different DNA probes. The binding was competed by poly(dI-dC), sonicated salmon sperm DNA, and circular or linearized plasmid DNA. The optimal DNA binding for the heterodimer was at pH 7-9 with low salt. The DNA-binding subunit of the heterodimer was the p100 polypeptide, as shown by u.v.-cross-linking assays and Southwestern blots.
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