ArticleThe Biochemical journal1993
Proteolytic removal of three C-terminal residues of actin alters the monomer-monomer interactions.
Article in The Biochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Actin's functional "switch": Constraining C-terminal conformational flexibility disrupts functionally important communication networks.The Journal of biological chemistry · 2026Article
- Acetylation of fission yeast tropomyosin does not promote differential association with cognate formins.Cytoskeleton (Hoboken, N.J.) · 2023Article
- Regulation of Actin Filament Length by Muscle Isoforms of Tropomyosin and Cofilin.International journal of molecular sciences · 2020Article
- Tropomyosin isoforms regulate cofilin 1 activity by modulating actin filament conformation.Archives of biochemistry and biophysics · 2020Article
- ATP and ADP actin states.Biopolymers · 2013Article
- F-actin structure destabilization and DNase I binding loop: fluctuations mutational cross-linking and electron microscopy analysis of loop states and effects on F-actin.Journal of molecular biology · 2010Article
- Involvement of S-nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide.Molecular pain · 2009Article
- Effect of actin C-terminal modification on tropomyosin isoforms binding and thin filament regulation.Biochimica et biophysica acta · 2009Article
- Functional effects of nemaline myopathy mutations on human skeletal alpha-actin.The Journal of biological chemistry · 2008Article
- Role of actin C-terminus in regulation of striated muscle thin filament.Biophysical journal · 2008Article
- The open nucleotide pocket of the profilin/actin x-ray structure is unstable and closes in the absence of profilin.Biophysical journal · 2006Article
- Solution properties of tetramethylrhodamine-modified G-actin.Biophysical journal · 2003Article
- Role of the DNase-I-binding loop in dynamic properties of actin filament.Biophysical journal · 2002Article
- The oxidation produced by hydrogen peroxide on Ca-ATP-G-actin.Protein science : a publication of the Protein Society · 2000Article
- Divalent cation-, nucleotide-, and polymerization-dependent changes in the conformation of subdomain 2 of actin.Biophysical journal · 1999Article
- Differential epitope tagging of actin in transformed Drosophila produces distinct effects on myofibril assembly and function of the indirect flight muscle.Molecular biology of the cell · 1999Article
- Structural changes in subdomain 2 of G-actin observed by fluorescence spectroscopy.The Biochemical journal · 1996Article
- Effects of the type of divalent cation, Ca2+ or Mg2+, bound at the high-affinity site and of the ionic composition of the solution on the structure of F-actin.The Biochemical journal · 1996Article
- H2O2-treated actin: assembly and polymer interactions with cross-linking proteins.Biophysical journal · 1995Article
- Long-range conformational effects of proteolytic removal of the last three residues of actin.The Biochemical journal · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homogeneous preparations of actin devoid of the three C-terminal residues were obtained by digestion of G-actin with trypsin after blocking proteolysis at other sites by substitution of Mg2+ for the tightly bound Ca2+. Removal of the C-terminal residues resulted in the following: an enhancement of the Mg(2+)-induced hydrolysis of ATP in low-ionic-strength solutions of actin; an increase in the critical concentration for polymerization; a decrease in the initial rate of polymerization; and an enhancement of the steady-state exchange of subunits in the polymer. Electron microscopy indicated an increased fragility of the filaments assembled from truncated actin. The results suggest that removal of the C-terminal residues increases the rate constants for monomer dissociation from the polymer ends and from the oligomeric species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.