ArticleThe Journal of membrane biology1993
Relation between cytoskeleton, hypo-osmotic treatment and volume regulation in Ehrlich ascites tumor cells.
Article in The Journal of membrane biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 83 citations in OpenAlex.
- Article
- The septin cytoskeleton facilitates membrane retraction during motility and blebbing.The Journal of cell biology · 2012Article
- Nitric oxide release follows endothelial nanomechanics and not vice versa.Pflugers Archiv : European journal of physiology · 2010Article
- Influence of hypotonic shock on glutamate and GABA uptake in rat brain synaptosomes.Neurochemical research · 2004Article
- Volume regulatory responses of basolateral membrane vesicles from Necturus enterocytes: role of the cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Modulation of glioma cell migration and invasion using Cl(-) and K(+) ion channel blockers.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- The role of swelling-induced anion channels during neuronal volume regulation.Molecular neurobiology · 1996Review
- Activation of the osmo-sensitive chloride conductance involves P21rho and is accompanied by a transient reorganization of the F-actin cytoskeleton.Molecular biology of the cell · 1996Article
- Actin Filaments in Mature Guard Cells Are Radially Distributed and Involved in Stomatal Movement.Plant physiology · 1995Article
- Hypotonically activated chloride current in HSG cells.The Journal of membrane biology · 1994Article
- Morphological alterations and cytoskeletal reorganization in opossum kidney (OK) cells during osmotic swelling and volume regulation.Histochemistry · 1994Article
- The Na-K-2Cl cotransporter is in a permanently activated state in cytoplasts from Ehrlich ascites tumor cells.The Journal of membrane biology · 1994Article
- Volume regulation in human fibroblasts: role of Ca2+ and 5-lipoxygenase products in the activation of the Cl- efflux.The Journal of membrane biology · 1993Article
- Cytoskeleton and ion movements during volume regulation in cultured PC12 cells.The Journal of membrane biology · 1993Article
- Na+, K+, Cl- cotransport and its regulation in Ehrlich ascites tumor cells. Ca2+/calmodulin and protein kinase C dependent pathways.The Journal of membrane biology · 1993Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pretreatment with cytochalasin B, which is known to disrupt microfilaments, significantly inhibits regulatory volume decrease (RVD) in Ehrlich ascites tumor cells, suggesting that an intact microfilament network is a prerequisite for a normal RVD response. Colchicine, which is known to disrupt microtubules, has no significant effect on RVD. Ehrlich cells have a cortical three-dimensional, orthogonal F-actin filament network which makes the cells look completely black in light microscopy following immunogold/silver staining using anti-actin antibodies. After addition of cytochalasin B, the stained cells get lighter with black dots localized to the plasma membrane and appearance of multiple knobby protrusions at cell periphery. Also, a significant decrease in the staining of the cells is seen after 15 min of RVD in hypotonic medium. This microfilament reorganization appears during RVD in the presence of external Ca2+ or Ca(2+)-ionophore A23187. It is, however, abolished in the absence of extracellular calcium, with or without prior depletion of intracellular Ca2+ stores. An effect of increased calcium influx might therefore be considered. The microfilament reorganization during RVD is abolished by the calmodulin antagonists pimozide and trifluoperazine, suggesting the involvement of calmodulin in the process. The microfilament reorganization is also prevented by addition of quinine. This quinine inhibition is overcome by addition of the K+ ionophore valinomycin.
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