ArticleJournal of bacteriology1993
Analysis of feedback-resistant anthranilate synthases from Saccharomyces cerevisiae.
Article in Journal of bacteriology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- OsASA2 Loss-of-Function Confers Broad-Spectrum Disease Resistance and Triggers Cell Death by Regulating Anthranilate Synthase Activity in Rice.Molecular plant pathology · 2026Article
- High Baseline Plasma Anthranilic Acid Predicts Remission Upon Acute-Series Ketamine Infusion for Treatment-Resistant Depression.Biological psychiatry global open science · 2025Article
- Metabolic Engineering of a Serotonin Overproducing Saccharomyces cerevisiae Strain.Microbial biotechnology · 2025Article
- Engineering the L-tryptophan metabolism for efficient de novo biosynthesis of tryptophol in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2024Article
- A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities.Nature microbiology · 2024Article
- Engineering of Saccharomyces cerevisiae for anthranilate and methyl anthranilate production.Microbial cell factories · 2021Article
- Combining mechanistic and machine learning models for predictive engineering and optimization of tryptophan metabolism.Nature communications · 2020Article
- Metabolic engineering of Saccharomyces cerevisiae for the de novo production of psilocybin and related tryptamine derivatives.Metabolic engineering · 2020Article
- A Multifaceted Role of Tryptophan Metabolism and Indoleamine 2,3-Dioxygenase Activity inFrontiers in immunology · 2017Review
- TrpE feedback mutants reveal roadblocks and conduits toward increasing secondary metabolism in Aspergillus fumigatus.Fungal genetics and biology : FG & B · 2016Article
- Genetic drift opposes mutualism during spatial population expansion.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Characterization of the altered anthranilate synthase in 5-methyltryptophan-resistant rice mutants.Plant cell reports · 2005Article
- Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathway.Plant physiology · 2005Article
- In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the alpha subunits OASA1 and OASA2.Plant molecular biology · 2004Article
- Characterization of rice anthranilate synthase alpha-subunit genes OASA1 and OASA2. Tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASA1.Plant physiology · 2001Article
- Coevolution of transcriptional and allosteric regulation at the chorismate metabolic branch point of Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Tissue culture-specific expression of a naturally occurring tobacco feedback-insensitive anthranilate synthase.Plant physiology · 1998Article
- Anthranilate synthase from Ruta graveolens. Duplicated AS alpha genes encode tryptophan-sensitive and tryptophan-insensitive isoenzymes specific to amino acid and alkaloid biosynthesis.Plant physiology · 1996Article
- The Arabidopsis thaliana trp5 mutant has a feedback-resistant anthranilate synthase and elevated soluble tryptophan.Plant physiology · 1996Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initial step of tryptophan biosynthesis is catalyzed by the enzyme anthranilate synthase, which in most microorganisms is subject to feedback inhibition by the end product of the pathway. We have characterized the TRP2 gene from a mutant Saccharomyces cerevisiae strain coding for an anthranilate synthase that is unresponsive to tryptophan. Sequence analysis of this TRP2(Fbr) (feedback-resistant) allele revealed numerous differences from a previously published TRP2 sequence. However, TRP2(Fbr) was found to differ in only one single-point mutation from its own parent wild type, a C-to-T transition resulting in a serine 76-to-leucine 76 amino acid substitution. Therefore, serine 76 is a crucial amino acid for proper regulation of the yeast enzyme. We constructed additional feedback-resistant enzyme forms of the yeast anthranilate synthase by site-directed mutagenesis of the conserved LLES sequence in the TRP2 gene. From analysis of these variants, we propose an extended sequence, LLESX10S, as the regulatory element in tryptophan-responsive anthranilate synthases from prokaryotic and eukaryotic organisms.
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