ArticleProceedings of the National Academy of Sciences of the United States of America1993
Low-frequency chimeric yeast artificial chromosome libraries from flow-sorted human chromosomes 16 and 21.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Flow karyotypes and chromosomal DNA contents of genus Triticum species and rye (Secale cereale).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Article
- Article
- Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1999Article
- Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- High-resolution flow karyotyping and chromosome sorting in Vicia faba lines with standard and reconstructed karyotypes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
- Physical structure of human chromosome 21: an analysis of YACs spanning 21q.Mammalian genome : official journal of the International Mammalian Genome Society · 1995Article
- The role of recombination and RAD52 in mutation of chromosomal DNA transformed into yeast.Nucleic acids research · 1994Article
- A mouse monochromosome 8 somatic cell hybrid: a reagent for chromosome 8 isolation.Mammalian genome : official journal of the International Mammalian Genome Society · 1994Article
- Characterization of four human YAC libraries for clone size, chimerism and X chromosome sequence representation.Nucleic acids research · 1994Article
- The P1 cloning system: past and future.Mammalian genome : official journal of the International Mammalian Genome Society · 1994Review
- Efficient identification and regional positioning of YAC and cosmid clones to human chromosome 21 by radiation fusion hybrids.Mammalian genome : official journal of the International Mammalian Genome Society · 1994Article
- Chimeric YACs were generated at unreduced rates in conditions that suppress coligation.Nucleic acids research · 1994Article
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4 authors.
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Abstract
Construction of chromosome-specific yeast artificial chromosome (YAC) libraries from sorted chromosomes was undertaken (i) to eliminate drawbacks associated with first-generation total genomic YAC libraries, such as the high frequency of chimeric YACs, and (ii) to provide an alternative method for generating chromosome-specific YAC libraries in addition to isolating such collections from a total genomic library. Chromosome-specific YAC libraries highly enriched for human chromosomes 16 and 21 were constructed. By maximizing the percentage of fragments with two ligatable ends and performing yeast transformations with less than saturating amounts of DNA in the presence of carrier DNA, YAC libraries with a low percentage of chimeric clones were obtained. The smaller number of YAC clones in these chromosome-specific libraries reduces the effort involved in PCR-based screening and allows hybridization methods to be a manageable screening approach.
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