ArticleThe EMBO journal1993
Intermediates in extrachromosomal homologous recombination in Xenopus laevis oocytes: characterization by electron microscopy.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
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- Establishment of a Transformation CoupledFrontiers in oncology · 2020Article
- Efficiency of nonhomologous DNA end joining varies among somatic tissues, despite similarity in mechanism.Cellular and molecular life sciences : CMLS · 2011Article
- Nonhomologous DNA end joining in cell-free extracts.Journal of nucleic acids · 2010Article
- The nature of the 5'-terminus is a major determinant for DNA processing by Schizosaccharomyces pombe Rad2p, a FEN-1 family nuclease.Nucleic acids research · 2000Article
- Regulated formation of extrachromosomal circular DNA molecules during development in Xenopus laevis.Molecular and cellular biology · 1999Article
- Methanococcus jannaschii flap endonuclease: expression, purification, and substrate requirements.Journal of bacteriology · 1998Article
- Processing of targeted psoralen cross-links in Xenopus oocytes.Molecular and cellular biology · 1997Article
- Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Complete DNA sequence of the mitochondrial genome of the black chiton, Katharina tunicata.Genetics · 1994Article
- Repair of heteroduplex DNA in Xenopus laevis oocytes.Genetics · 1994Article
- Distribution of exchanges upon homologous recombination of exogenous DNA in Xenopus laevis oocytes.Genetics · 1994Article
- The characterization of a mammalian DNA structure-specific endonuclease.The EMBO journal · 1994Article
- Illegitimate recombination in Xenopus: characterization of end-joined junctions.Nucleic acids research · 1994Article
- Homologous and illegitimate recombination in developing Xenopus oocytes and eggs.Molecular and cellular biology · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Several molecular mechanisms have been proposed to account for nonconservative homologous recombination. This type of recombination is particularly efficient in Xenopus oocytes when appropriate DNA substrates are injected. To distinguish between possible models, we have investigated recombination intermediates from oocytes by direct observation in the electron microscope. Partially recombined DNA was crosslinked with a psoralen derivative after incubation in oocytes to limit the branch migration that might occur during recovery procedures and alter the structures that were initially present. Branched structures, which we interpret as intermediates, represented approximately 10% of the DNA recovered and were readily analyzed. We did not observe any structures with internal loops predicted by invasion mechanisms. The majority of intermediates had one or two single-stranded branches on product-sized molecules, as predicted for incomplete junctions in the resection-annealing mechanism. Detailed length measurements confirmed the expectations of that model. When recovered DNA was not crosslinked, or when annealed junctions were prepared in vitro, we saw branched structures that indicated the occurrence of extensive branch migration. Comparison with the crosslinked sample confirmed the effectiveness of the crosslinking in preserving structures created in the oocytes. Our results strongly support a resection-annealing mechanism of recombination in oocytes.
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