ArticleThe EMBO journal1993
Oct-2 facilitates functional preinitiation complex assembly and is continuously required at the promoter for multiple rounds of transcription.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 47 citations in OpenAlex.
- BCL-W is dispensable for the sustained survival of select Burkitt lymphoma and diffuse large B-cell lymphoma cell lines.Blood advances · 2020Article
- Characterization of cis-acting elements required for autorepression of the equine herpesvirus 1 IE gene.Virus research · 2012Article
- Herpes simplex virus 1 ICP4 forms complexes with TFIID and mediator in virus-infected cells.Journal of virology · 2011Article
- Activity of metal-responsive transcription factor 1 by toxic heavy metals and H2O2 in vitro is modulated by metallothionein.Molecular and cellular biology · 2003Article
- Direct association of Bloom's syndrome gene product with the human mismatch repair protein MLH1.Nucleic acids research · 2001Article
- Herpes simplex virus type 1 ICP4 promotes transcription preinitiation complex formation by enhancing the binding of TFIID to DNA.Journal of virology · 2000Article
- Efficient transcription of an immunoglobulin kappa promoter requires specific sequence elements overlapping with and downstream of the transcriptional start site.Nucleic acids research · 1997Article
- POU domain transcription factors in embryonic development.Molecular biology reports · 1997Review
- Transcription reinitiation rate: a special role for the TATA box.Molecular and cellular biology · 1997Article
- Stimulation of basal transcription from the mouse mammary tumor virus promoter by Oct proteins.Journal of virology · 1995Article
- Sarkosyl block of transcription reinitiation by RNA polymerase II as visualized by the colliding polymerases reinitiation assay.Nucleic acids research · 1994Article
- Identification of functional targets of the Zta transcriptional activator by formation of stable preinitiation complex intermediates.Molecular and cellular biology · 1994Article
- YY1 represses rat serum amyloid A1 gene transcription and is antagonized by NF-kappa B during acute-phase response.Molecular and cellular biology · 1994Article
- The POU domains of the Oct1 and Oct2 transcription factors mediate specific interaction with TBP.Nucleic acids research · 1994Article
- Two different IFN-gamma nonresponsive variants derived from the B-cell lymphoma 70Z/3.Immunogenetics · 1994Article
- Specific transcriptional activation in vitro by the herpes simplex virus protein VP16.Nucleic acids research · 1993Article
- Function of the growth-regulated transcription initiation factor TIF-IA in initiation complex formation at the murine ribosomal gene promoter.Molecular and cellular biology · 1993Article
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4 authors at 1 institution in 1 country.
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Abstract
Octamer factor 2 (Oct-2, OTF-2, NF-A2) is an 'upstream' promoter factor that binds to the octamer motif (ATGCAAAT) implicated in control of immunoglobulin gene transcription in B-lymphocytes. We have studied the role of Oct-2 in the process of transcription initiation in vitro using both nuclear extracts and purified basal transcription factors. Oct-2 specifically stimulates transcription from octamer-containing promoters in both systems. Thus, Oct-2 is a 'true activator', rather than merely an 'anti-repressor' counteracting the effect of histones. In order-of-addition experiments, Oct-2 is required early, together with TFIID, to allow formation of a preinitiation complex. Oct-2 cannot functionally interact with cloned TATA binding protein (TBP) but rather requires 'coactivators' found in the TFIID fraction. In single-round transcription experiments, early competition for Oct-2 by an octamer oligonucleotide is deleterious, but no effect is seen after assembly of a complete preinitiation complex. However, for multiple rounds of transcription, Oct-2 is continuously required at the promoter; this result argues against a 'hit-and-run' mechanism whereby the activator becomes dispensible after organizing a TFIID-promoter complex. In agreement with our previous studies in vivo, the N-terminal glutamine-rich activation domain of Oct-2 is required for full activity in vitro, indicating that this domain directly interacts with basal transcription factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.