ArticleThe Journal of experimental medicine1993
Somatic hypermutation of an immunoglobulin mu heavy chain transgene.
Article in The Journal of experimental medicine, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 68 citations in OpenAlex.
- Elucidation of IgH intronic enhancer functions via germ-line deletion.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A hallmark of active class switch recombination: transcripts directed by I promoters on looped-out circular DNAs.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Induction of Ig somatic hypermutation and class switching in a human monoclonal IgM+ IgD+ B cell line in vitro: definition of the requirements and modalities of hypermutation.Journal of immunology (Baltimore, Md. : 1950) · 1999Article
- The promotion of V region hypermutation.The Journal of experimental medicine · 1997Article
- The transcriptional promoter regulates hypermutation of the antibody heavy chain locus.The Journal of experimental medicine · 1997Article
- Differential V region mutation of two transfected Ig genes and their interaction in cultured B cell lines.The EMBO journal · 1996Article
- Comparison of somatic mutation frequency among immunoglobulin genes.The Journal of experimental medicine · 1994Article
- Somatic generation of hybrid antibody H chain genes in transgenic mice via interchromosomal gene conversion.The Journal of experimental medicine · 1994Article
- Analysis of somatic hypermutation in mouse Peyer's patches using immunoglobulin kappa light-chain transgenes.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have analyzed somatic hypermutation of an immunoglobulin (Ig) heavy chain transgene. Hybridomas expressing the transgene were produced from immunized transgenic mice and transgene copies were sequenced to assay for mutation. In two IgM-producing hybridomas, as well as in several IgG-producing hybridomas, mutations were found in the VDJ region of the transgene. In the IgM-producing hybridomas, both mutated and unmutated transgene copies were present and expressed as mRNA. Several mutated transgene copies were present in a single cell and these showed different patterns of mutation. Two IgG-producing hybridomas isolated from a single animal also showed a hierarchical pattern of mutation indicating that transgene mutations can accumulate during B cell proliferation, similar to the mutational process for endogenous antibody genes. Among hybridomas that expressed both IgG and IgM molecules derived from the transgene, the isotype-switched gamma transgene copy exhibited a higher level of mutation than the mu transgene copies. Our results indicate that the 15-kb ARSmu transgene contains all the sequence information required to target the Ig-specific hypermutational machinery, and raise the possibility that sequences associated with the endogenous CH locus might enhance somatic mutation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.