ArticleThe Journal of cell biology1993
Elastic filaments in situ in cardiac muscle: deep-etch replica analysis in combination with selective removal of actin and myosin filaments.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 119 citations in OpenAlex.
- Nanoscopic changes in the lattice structure of striated muscle sarcomeres involved in the mechanism of spontaneous oscillatory contraction (SPOC).Scientific reports · 2020Article
- An historical perspective of the discovery of titin filaments -Part 2.Biophysical reviews · 2018Review
- Spontaneous oscillatory contraction (SPOC) in cardiomyocytes.Biophysical reviews · 2015Article
- Is titin a 'winding filament'? A new twist on muscle contraction.Proceedings. Biological sciences · 2012Article
- Sarcomere imaging by quantum dots for the study of cardiac muscle physiology.Journal of biomedicine & biotechnology · 2012Review
- SPontaneous Oscillatory Contraction (SPOC): auto-oscillations observed in striated muscle at partial activation.Biophysical reviews · 2011Review
- Titin-actin interaction: PEVK-actin-based viscosity in a large animal.Journal of biomedicine & biotechnology · 2011Article
- Thin filament-reconstituted skinned muscle fibers for the study of muscle physiology.Journal of biomedicine & biotechnology · 2011Review
- Titin-isoform dependence of titin-actin interaction and its regulation by S100A1/Ca2+ in skinned myocardium.Journal of biomedicine & biotechnology · 2010Article
- Roles of titin in the structure and elasticity of the sarcomere.Journal of biomedicine & biotechnology · 2010Review
- Loss of Smyhc1 or Hsp90alpha1 function results in different effects on myofibril organization in skeletal muscles of zebrafish embryos.PloS one · 2010Article
- Titin and troponin: central players in the frank-starling mechanism of the heart.Current cardiology reviews · 2009Article
- New aspects of obscurin in human striated muscles.Histochemistry and cell biology · 2008Article
- Use of thin filament reconstituted muscle fibres to probe the mechanism of force generation.Journal of muscle research and cell motility · 2006Review
- Can the passive elasticity of muscle be explained directly from the mechanics of individual titin molecules?Journal of muscle research and cell motility · 2005Article
- Plasticity of cardiac titin/connectin in heart development.Journal of muscle research and cell motility · 2005Article
- Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1.The Journal of cell biology · 2002Article
- Titin-based contribution to shortening velocity of rabbit skeletal myofibrils.The Journal of physiology · 2002Article
- Role of titin in vertebrate striated muscle.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2002Review
- Temperature effect on isometric tension is mediated by regulatory proteins tropomyosin and troponin in bovine myocardium.The Journal of physiology · 2002Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To clarify the full picture of the connectin (titin) filament network in situ, we selectively removed actin and myosin filaments from cardiac muscle fibers by gelsolin and potassium acetate treatment, respectively, and observed the residual elastic filament network by deep-etch replica electron microscopy. In the A bands, elastic filaments of uniform diameter (6-7 nm) projecting from the M line ran parallel, and extended into the I bands. At the junction line in the I bands, which may correspond to the N2 line in skeletal muscle, individual elastic filaments branched into two or more thinner strands, which repeatedly joined and branched to reach the Z line. Considering that cardiac muscle lacks nebulin, it is very likely that these elastic filaments were composed predominantly of connectin molecules; indeed, anti-connectin monoclonal antibody specifically stained these elastic filaments. Further, striations of approximately 4 nm, characteristic of isolated connectin molecules, were also observed in the elastic filaments. Taking recent analyses of the structure of isolated connectin molecules into consideration, we concluded that individual connectin molecules stretched between the M and Z lines and that each elastic filament consisted of laterally-associated connectin molecules. Close comparison of these images with the replica images of intact and S1-decorated sarcomeres led us to conclude that, in intact sarcomeres, the elastic filaments were laterally associated with myosin and actin filaments in the A and I bands, respectively. Interestingly, it was shown that the elastic property of connectin filaments was not restricted by their lateral association with actin filaments in intact sarcomeres. Finally, we have proposed a new structural model of the cardiac muscle sarcomere that includes connectin filaments.
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