ArticleThe Journal of clinical investigation1993
Band 3 and glycophorin are progressively aggregated in density-fractionated sickle and normal red blood cells. Evidence from rotational and lateral mobility studies.
Article in The Journal of clinical investigation, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- Microfluidic study of retention and elimination of abnormal red blood cells by human spleen with implications for sickle cell disease.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
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- Membrane protein dynamics and functional implications in mammalian cells.Current topics in membranes · 2013Article
- Article
- Membrane compartmentalization in Southeast Asian ovalocytosis red blood cells.British journal of haematology · 2011Article
- Band 3 catalyzes sickle hemoglobin polymerization.Biophysical chemistry · 2010Article
- C3b deposition on human erythrocytes induces the formation of a membrane skeleton-linked protein complex.The Journal of clinical investigation · 2009Article
- Soft tissue and cellular preservation in vertebrate skeletal elements from the Cretaceous to the present.Proceedings. Biological sciences · 2007Article
- Binding of hemoglobin to red cell membranes with eosin-5-maleimide-labeled band 3: analysis of centrifugation and fluorescence data.Biochemistry · 2002Article
- Flexibility of the cytoplasmic domain of the anion exchange protein, band 3, in human erythrocytes.Biophysical journal · 2001Article
- Membrane dynamics of the water transport protein aquaporin-1 in intact human red cells.Biophysical journal · 1999Article
- Oligomeric state of human erythrocyte band 3 measured by fluorescence resonance energy homotransfer.Biophysical journal · 1998Article
- The orientation of eosin-5-maleimide on human erythrocyte band 3 measured by fluorescence polarization microscopy.Biophysical journal · 1996Article
- Control of band 3 lateral and rotational mobility by band 4.2 in intact erythrocytes: release of band 3 oligomers from low-affinity binding sites.Biophysical journal · 1996Article
- Red cell membrane remodeling in sickle cell anemia. Sequestration of membrane lipids and proteins in Heinz bodies.The Journal of clinical investigation · 1996Article
- Analysis of saturation transfer electron paramagnetic resonance spectra of a spin-labeled integral membrane protein, band 3, in terms of the uniaxial rotational diffusion model.Biophysical journal · 1995Article
- Millisecond measurement of transport during and after an electroporation pulse.Biophysical journal · 1995Article
- Exercise, training and red blood cell turnover.Sports medicine (Auckland, N.Z.) · 1995Review
- Differential control of band 3 lateral and rotational mobility in intact red cells.The Journal of clinical investigation · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Band 3 aggregation in the plane of the red blood cell (RBC) membrane is postulated to be important in the pathophysiology of hemolysis of dense sickle and normal RBCs. We used the fluorescence photobleaching recovery and polarized fluorescence depletion techniques to measure the lateral and rotational mobility of band 3, glycophorins, and phospholipid analogues in membranes of density-separated intact RBCs from seven patients with sickle cell disease and eight normal controls. The fractions of laterally mobile band 3 and glycophorin decreased progressively as sickle RBC density increased. Normal RBCs also showed a progressive decrease in band 3 fractional mobility with increasing buoyant density. Rapidly rotating, slowly rotating, and rotationally immobile forms of band 3 were observed in both sickle and normal RBC membranes. The fraction of rapidly rotating band 3 progressively decreased and the fraction of rotationally immobile band 3 progressively increased with increasing sickle RBC density. Changes in the fraction of rotationally immobile band 3 were not reversible upon hypotonic swelling of dense sickle RBCs, and normal RBCs osmotically shrunken in sucrose buffers failed to manifest band 3 immobilization at median cell hemoglobin concentration values characteristic of dense sickle RBCs. We conclude that dense sickle and normal RBCs acquire irreversible membrane abnormalities that cause transmembrane protein immobilization and band 3 aggregation. Band 3 aggregates could serve as cell surface sites of autologous antibody binding and thereby lead to removal of dense sickle and normal (senescent) RBCs from the circulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.