ArticleInfection and immunity1993
Light emission from a Mudlux transcriptional fusion in Salmonella typhimurium is stimulated by hydrogen peroxide and by interaction with the mouse macrophage cell line J774.2.
Article in Infection and immunity, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Aminoglycosides affect intracellular Salmonella enterica serovars typhimurium and virchow.Antimicrobial agents and chemotherapy · 2008Article
- Evolution, adaptation, and supercoiling.Journal of bacteriology · 2007Article
- Monitoring bioluminescent Staphylococcus aureus infections in living mice using a novel luxABCDE construct.Infection and immunity · 2000Article
- DNA topology and adaptation of Salmonella typhimurium to an intracellular environment.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- Measurement of bacterial gene expression in vivo.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- The Salmonella typhimurium AhpC polypeptide is not essential for virulence in BALB/c mice but is recognized as an antigen during infection.Infection and immunity · 1998Article
- Identification of the ahp operon of Salmonella typhimurium as a macrophage-induced locus.Journal of bacteriology · 1997Article
- DNA repair is more important than catalase for Salmonella virulence in mice.The Journal of clinical investigation · 1995Article
- Intracellular generation of superoxide as a by-product of Vibrio harveyi luciferase expressed in Escherichia coli.Journal of bacteriology · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen peroxide is known to induce a multigenic response in Salmonella typhimurium cells. We have used a Mudlux transcriptional reporter system to identify and isolate fusions in the virulent strain SL1344 which respond to hydrogen peroxide in vitro by light production, and one of these fusions, MPG203, has been further characterized. Transient light production was observed from MPG203 at levels of hydrogen peroxide as low as 10 microM. However, high levels of this toxic oxidizing agent resulted in light suppression, particularly at low bacterial densities. This fusion was also shown to produce light following adhesion to cells of the mouse macrophage cell line J774.2. Furthermore, the response was greatly reduced in the presence of catalase, directly implicating hydrogen peroxide as the eliciting agent and suggesting the involvement of the hydrogen peroxide-induced bacterial stress response in the infection process. Chemiluminescence studies also indicated that inhibition of the respiratory burst may occur as the infection ratio is increased. In addition, the level of light produced from bacteria within individual macrophage cells was shown to vary.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.