ArticleProceedings of the National Academy of Sciences of the United States of America1993
Inhibitory effects of antisense oligodeoxynucleotides targeting c-myc mRNA on smooth muscle cell proliferation and migration.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Targeted Downregulation of MYC through G-quadruplex Stabilization by DNAi.Molecules (Basel, Switzerland) · 2021Article
- Gene polymorphisms in the PI3K/AKT/mTOR signaling pathway contribute to prostate cancer susceptibility in Chinese men.Oncotarget · 2017Article
- All-Atom MD Predicts Magnesium-Induced Hairpin in Chemically Perturbed RNA Analog of F10 Therapeutic.The journal of physical chemistry. B · 2017Article
- Nucleic acid clamp-mediated recognition and stabilization of the physiologically relevant MYC promoter G-quadruplex.Nucleic acids research · 2016Article
- Delivery of oligonucleotides with lipid nanoparticles.Advanced drug delivery reviews · 2015Review
- Treatment of chronic proliferative cholangitis with c-myc shRNA.World journal of gastroenterology · 2009Article
- Incorporation of two modified nucleosides allows selective platination of an oligonucleotide making it suitable for duplex cross-linking.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2007Article
- Novel c-MYC target genes mediate differential effects on cell proliferation and migration.EMBO reports · 2007Article
- Molecular pathogenesis of chronic wounds: the role of beta-catenin and c-myc in the inhibition of epithelialization and wound healing.The American journal of pathology · 2005Article
- A genetic screen to identify genes that rescue the slow growth phenotype of c-myc null fibroblasts.Oncogene · 2000Article
- An in vivo function for the transforming Myc protein: elicitation of the angiogenic phenotype.Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research · 2000Article
- Targeted expression of c-Myc in the epidermis alters normal proliferation, differentiation and UV-B induced apoptosis.Oncogene · 1999Article
- Tissue-selective expression of dominant-negative proteins for the regulation of vascular smooth muscle cell proliferation.Gene therapy · 1999Article
- Gi-Protein alpha-subunit mRNA antisense oligonucleotide inhibition of Gi-coupled receptor contractile activity in the epididymis of the guinea-pig.British journal of pharmacology · 1999Article
- Inhibition of B16BL6 melanoma invasion by tyrosine and phenylalanine deprivation is associated with decreased secretion of plasminogen activators and increased plasminogen activator inhibitors.Clinical & experimental metastasis · 1999Article
- Activation of the MAP kinase pathway by FGF-1 correlates with cell proliferation induction while activation of the Src pathway correlates with migration.The Journal of cell biology · 1998Article
- Phosphorothioate Oligodeoxynucleotide Inhibition of Restenosis.Journal of thrombosis and thrombolysis · 1998Article
- Contiguous four-guanosine sequence in c-myc antisense phosphorothioate oligonucleotides inhibits cell growth on human lung cancer cells: possible involvement of cell adhesion inhibition.Japanese journal of cancer research : Gann · 1997Article
- Consensus-interferon and platelet-derived growth factor adversely regulate proliferation and migration of Kaposi's sarcoma cells by control of c-myc expression.The American journal of pathology · 1996Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Smooth muscle cell (SMC) proliferation and migration play pivotal roles in restenosis following angioplasty. c-myc is an immediate early response gene induced by various mitogens, and several lines of evidence derived from experiments using transformed or hematopoietic cell lines, or transgenic mice, suggest its protein product plays a role in numerous signaling transduction pathways, including those modulating cell division. We therefore reasoned that a strategy employing oligodeoxynucleotides (ODNs) complementary to c-myc mRNA (antisense ODNs) might be potent inhibitors of SMC proliferation and, perhaps, of SMC migration. To evaluate this concept, we tested several antisense ODNs targeted to c-myc mRNA (15- or 18-mer ODNs complementary to different c-myc mRNA sequences) by introducing them individually into the medium of cultured rat aortic SMCs. Phosphoroamidate-modified ODNs were employed to retard degradation. Antisense ODNs inhibited, in a concentration-dependent manner, SMC proliferation and SMC migration. Maximal inhibitory effect was 50% for proliferation and > 90% for migration. These effects were associated with decreased SMC expression of c-myc-encoded protein by Western immunoblotting and immunocytochemical staining. ODNs with the same nucleotides but a scrambled sequence caused no effect. These results indicate that the c-myc gene product is involved in the signal transduction pathways mediating SMC proliferation and migration in the in vitro model we employed. The results also suggest a potential role of antisense strategies designed to inhibit c-myc expression for the prevention of coronary restenosis.
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