ArticleProceedings of the National Academy of Sciences of the United States of America1993
Genome mapping by nonrandom anchoring: a discrete theoretical analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Mapping by sequencing the Pneumocystis genome using the ordering DNA sequences V3 tool.Genetics · 2003Article
- Article
- Theories and applications for sequencing randomly selected clones.Genome research · 2001Article
- Parking strategies for genome sequencing.Genome research · 2000Article
- Article
- In vitro reconstruction of the Aspergillus (= Emericella) nidulans genome.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- On the consistency of a physical mapping method to reconstruct a chromosome in vitro.Genetics · 1996Article
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Authors and funding
2 authors.
Funding
Abstract
As part of our effort to construct a physical map of the genome of the fission yeast Schizosaccharomyces pombe, we have made theoretical predictions for the progress expected, as measured by the expected length fraction of island coverage and by the expected properties of the anchored islands such as the number and the size of islands. Our experimental strategy is to construct a random clone library and screen the library for clones having unique sequence at both ends. This scheme is essentially the same as the clone-limited double sequence-tagged-site selection scheme which was used in a computer simulation by Palazzolo et al. [Palazzolo, M. J., Sawyer, S. A., Martin, C. H., Smoller, D. A. & Hartl, D. L. (1991) Proc. Natl. Acad. Sci. USA 88, 8034-8038]. Both simulation and ongoing experiments in our laboratory have shown that the nonrandom anchoring method is far superior to random anchoring. In this paper, we propose a theoretical model to explain the simulated data and the experimental data.
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Registered trials
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