ArticleThe Journal of cell biology1993
Monoclonal antibody 7H6 reacts with a novel tight junction-associated protein distinct from ZO-1, cingulin and ZO-2.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Multiple sclerosis: etiology in the context of neurovascular unit and immune system involvement and advancements withFrontiers in immunology · 2025Review
- The blood-brain barrier: structure, regulation, and drug delivery.Signal transduction and targeted therapy · 2023Review
- Tight Junction Proteins and the Biology of Hepatobiliary Disease.International journal of molecular sciences · 2020Review
- Expression of periaxin (PRX) specifically in the human cerebrovascular system: PDZ domain-mediated strengthening of endothelial barrier function.Scientific reports · 2018Article
- Tight junction-related barrier contributes to the electrophysiological asymmetry across vocal fold epithelium.PloS one · 2012Article
- Effect of cAMP derivates on assembly and maintenance of tight junctions in human umbilical vein endothelial cells.BMC cell biology · 2010Article
- High-glucose and advanced glycosylation end products increased podocyte permeability via PI3-K/Akt signaling.Journal of molecular medicine (Berlin, Germany) · 2010Article
- Cellular elements of the blood-brain barrier.Neurochemical research · 2009Review
- Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions.The Journal of biological chemistry · 2009Article
- Oxidative stress-induced disruption of epithelial and endothelial tight junctions.Frontiers in bioscience : a journal and virtual library · 2008Article
- Human astrocytes/astrocyte-conditioned medium and shear stress enhance the barrier properties of human brain microvascular endothelial cells.Brain research · 2007Article
- Proteomic and bioinformatic analysis of epithelial tight junction reveals an unexpected cluster of synaptic molecules.Biology direct · 2006Article
- The organization of tight junctions in epithelia: implications for mammary gland biology and breast tumorigenesis.Journal of mammary gland biology and neoplasia · 2003Review
- Endothelial barriers: from hypothetical pores to membrane proteins.Journal of anatomy · 2002Review
- Blood-brain barrier breakdown in septic encephalopathy and brain tumours.Journal of anatomy · 2002Review
- alpha-catenin-independent recruitment of ZO-1 to nectin-based cell-cell adhesion sites through afadin.Molecular biology of the cell · 2001Article
- OSP/claudin-11 forms a complex with a novel member of the tetraspanin super family and beta1 integrin and regulates proliferation and migration of oligodendrocytes.The Journal of cell biology · 2001Article
- Restoration of tight junction structure and barrier function by down-regulation of the mitogen-activated protein kinase pathway in ras-transformed Madin-Darby canine kidney cells.Molecular biology of the cell · 2000Article
- Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins.The Journal of cell biology · 1999Article
- Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cells.The Journal of cell biology · 1999Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tight junction is an essential element of the intercellular junctional complex; yet its protein composition is not fully understood. At present, only three proteins, ZO-1 (Stevenson, B. R., J. D. Siliciano, M. S. Mooseker, and D. A. Goodenough. 1986. J. Cell Biol. 103:755-766), cingulin (Citi, S., H. Sabanay, R. Jakes, B. Geiger, and J. Kendrick-Jones. 1988. Nature (Lond.). 333:272-275) and ZO-2 (Gumbiner, B., T. Lowenkopf, and D. Apatira. 1991. Proc. Natl. Acad. Sci. USA. 88:3460-3464) are known to be associated with the tight junction. We have generated a monoclonal antibody (7H6) against a bile canaliculus-rich membrane fraction prepared from rat liver. This 7H6 antigen was preferentially localized by immunofluorescence at the junctional complex regions of hepatocytes and other epithelia, and 7H6-affiliated gold particles were shown electron microscopically to localize at the periphery of tight junctions. Immunoblot analysis of a bile canaliculus-rich fraction of rat liver using 7H6, anti-ZO-1 antibody (R26.4C), and anti-cingulin antibody revealed that 7H6 reacted selectively with a 155-kD protein, whereas R26.4C reacted only with a 225-kD protein. Anti-cingulin antibody reacted solely with 140 and 108-kD proteins, indicating that the protein recognized by 7H6 is immunologically different from ZO-1 and cingulin. Immunoprecipitation of detergent extracts obtained from metabolically labeled MDCK cells with R26.4C coprecipitated a 160-kD protein, which corresponds to ZO-2, with ZO-1. However, 7H6 did not react with the 160-kD protein. These results strongly suggest that the 7H6 antibody recognizes a novel tight junction-associated protein different from ZO-1, cingulin and ZO-2.
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