ArticleThe Journal of cell biology1993
Dynamics of microtubules bundled by microtubule associated protein 2C (MAP2C).
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 58 citations in OpenAlex.
- PLK4 is a microtubule-associated protein that self-assembles promotingJournal of cell science · 2018Article
- Transcriptional upregulation of microtubule-associated protein 2 is involved in the protein kinase A-induced decrease in the invasiveness of glioma cells.Neuro-oncology · 2015Article
- SDF1 reduces interneuron leading process branching through dual regulation of actin and microtubules.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Direct observation of microtubule pushing by cortical dynein in living cells.Molecular biology of the cell · 2014Article
- Biochemistry and cell biology of tau protein in neurofibrillary degeneration.Cold Spring Harbor perspectives in medicine · 2012Review
- MAP2-mediated in vitro interactions of brain microtubules and their modulation by cAMP.European biophysics journal : EBJ · 2009Article
- The pre-mRNA-splicing factor SF3a66 functions as a microtubule-binding and -bundling protein.The Biochemical journal · 2004Article
- The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle.The Journal of cell biology · 1997Article
- Microtubule-associated protein 2c reorganizes both microtubules and microfilaments into distinct cytological structures in an actin-binding protein-280-deficient melanoma cell line.The Journal of cell biology · 1997Article
- Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis.The Journal of cell biology · 1996Article
- Juvenile and mature MAP2 isoforms induce distinct patterns of process outgrowth.Molecular biology of the cell · 1996Article
- Polarity orientation and assembly process of microtubule bundles in nocodazole-treated, MAP2c-transfected COS cells.Molecular biology of the cell · 1995Article
- Influence of phosphorylation on isoform composition and function of a microtubule-associated protein from developing Artemia.The Biochemical journal · 1995Article
- Bundling of microtubules in transfected cells does not involve an autonomous dimerization site on the MAP2 molecule.Molecular biology of the cell · 1994Article
- Molecular analysis of the INCENPs (inner centromere proteins): separate domains are required for association with microtubules during interphase and with the central spindle during anaphase.The Journal of cell biology · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAP2C is a microtubule-associated protein abundant in immature nerve cells. We isolated a cDNA clone encoding whole mouse MAP2C of 467 amino acid residues. In fibroblasts transiently transfected with cDNA of MAP2C, interphase microtubule networks were reorganized into microtubule bundles. To reveal the dynamic properties of microtubule bundles, we analyzed the incorporation sites of exogenously introduced tubulin by microinjection of biotin-labeled tubulin and the turnover rate of microtubule bundles by photoactivation of caged fluorescein-labeled tubulin. The injected biotin-labeled tubulin was rapidly incorporated into distal ends of preexisting microtubule bundles, suggesting a concentration of the available ends of microtubules at this region. Although homogenous staining of microtubule bundles with antibiotin antibody was observed 2 h after injection, the photoactivation study indicated that turnover of microtubule bundles was extremely suppressed and < 10% of tubulin molecules would be exchanged within 1 h. Multiple photoactivation experiments provided evidence that neither catastrophic disassembly at the distal ends of bundles nor concerted disassembly due to treadmilling at the proximal ends could explain the observed rapid incorporation of exogenously introduced tubulin molecules. We conclude that microtubules bundled by MAP2C molecules are very stable while the abrupt increase of free tubulin molecules by microinjection results in rapid assembly from the distal ends within the bundles as well as free nucleation of small microtubules which are progressively associated laterally with preexisting microtubule bundles. This is the first detailed study of the function of MAPs on the dynamics of microtubules in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.